外源赤霉素对盐胁迫下水稻种子萌发及幼苗生长的影响

乔  娇

doi:10.3969/j.issn.1009-7791.2024.06.003

亚热带植物科学 >

2024 , Vol. 53 >Issue 6: 512 - 519

DOI: https://doi.org/10.3969/j.issn.1009-7791.2024.06.003

研究论文

外源赤霉素对盐胁迫下水稻种子萌发及幼苗生长的影响

乔娇

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(山西电子科技学院，山西临汾 041000)

收稿日期: 2024-07-18

录用日期: 2024-08-19

网络出版日期: 2025-03-20

基金资助

山西省高等学校教学改革创新项目(J20231810)；山西省基础研究计划青年科学研究项目(202203021212390)；山西省科技厅，山西省基础研究计划（自由探索类）项目编号202203021212385；山西省高等学校教学改革创新项目（J20231827）

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Effects of Exogenous Gibberellin on Rice Seed Germination and Seedling Growth under Salt Stress

QIAO Jiao

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(Shanxi University of Electronic Science and Technology, Linfen 041000, Shanxi China)

Received date: 2024-07-18

Accepted date: 2024-08-19

Online published: 2025-03-20

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摘要

通过添加不同浓度赤霉素(GA₃，25、50、100 mg·L^–1)，探究其对氯化钠胁迫下水稻种子萌发和幼苗生长的调控作用。结果表明，在种子萌发阶段，外源赤霉素促进盐胁迫下水稻种子萌发，其发芽势、发芽率、发芽指数均上升，相对伤害率降低。在幼苗生长阶段，形态分析表明，外源赤霉素缓解盐胁迫对幼苗生长的抑制，株高、根长、根尖数及叶面积上升；生理生化分析表明，外源赤霉素提高盐胁迫下水稻叶片超氧化物歧化酶(SOD)活性、降低叶片丙二醛(MDA)含量，提高光合色素含量叶绿素a、b，及类胡萝卜素含量上升，光合作用增强PSⅡ有效量子产量[Y(Ⅱ)]和光化学猝灭系数(qP)上升，非光化学淬灭系数(NPQ)下降。综上所述，外源赤霉素浸种处理可显著促进盐胁迫下水稻种子萌发，喷洒外源赤霉素后氯化钠胁迫对幼苗生长发育的影响明显减弱，最适赤霉素浓度为50 mg·L^–1。该研究结果为优化盐渍地水稻种植提供理论依据。

关键词： 外源赤霉素; 盐胁迫; 水稻; 种子萌发; 幼苗生长

本文引用格式

乔娇 . 外源赤霉素对盐胁迫下水稻种子萌发及幼苗生长的影响[J]. 亚热带植物科学, 2024 , 53(6) : 512 -519 . DOI: 10.3969/j.issn.1009-7791.2024.06.003

Abstract

This experiment investigated the effects of different concentrations of gibberellin (25, 50, 100 mg·L^–1) on rice seed germination and seedling growth under sodium chloride stress. The results showed that during the seed germination stage, exogenous gibberellin enhances the germination of rice seeds under salt stress, with an increase in germination vigor, germination rate, and germination index, and a decrease in relative damage rate. During the growth stage of seedlings, morphological analysis showed that exogenous gibberellin alleviated the inhibition of salt stress on seedling growth, resulting in an increase in plant height, root length, root tip number, and leaf area; Physiological and biochemical analysis showed that exogenous gibberellin improved the activity of superoxide dismutase (SOD) in rice leaves under salt stress, reduced the content of malondialdehyde (MDA) in leaves, increased the content of photosynthetic pigments including chlorophyll a, b and carotenoids, enhanced photosynthesis, increased the effective quantum yield of PSⅡ [Y(Ⅱ)] and the photochemical quenching coefficient (qP), and decreased the non photochemical quenching coefficient (NPQ). In summary, exogenous gibberellin soaking treatment significantly promoted the germination of rice seeds under salt stress, the effects of sodium chloride stress on seedling growth and development was significantly reduced after spraying exogenous gibberellin, and the optimal concentration of gibberellin was 50 mg·L^–1. The research results provide a theoretical basis for optimizing rice cultivation in saline soil.

Key words： gibberellin; salt stress; rice; seed germination; seedling growth

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