多主棒孢霉对不同品种烟草的致病性及其培养条件优化研究

HUANG Chun-yang; YANG Xiang; ZHOU Hong-jiang; SHI Cai-hua; WANG Han-cheng; HU Jing-rong

doi:10.3969/j.issn.1009-7791.2025.01.003

亚热带植物科学 >

2025 , Vol. 54 >Issue 1: 22 - 28

DOI: https://doi.org/10.3969/j.issn.1009-7791.2025.01.003

研究论文

多主棒孢霉对不同品种烟草的致病性及其培养条件优化研究

HUANG Chun-yang ,
YANG Xiang ,
ZHOU Hong-jiang ,
SHI Cai-hua ,
WANG Han-cheng ,
HU Jing-rong

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(1. 贵州省烟草公司遵义分公司，遵义贵州 563000；2. 湖北文理学院现代农业研究院，襄阳湖北 441054；3. 贵州省烟草科学研究院，贵阳贵州 550081)

收稿日期: 2024-07-20

录用日期: 2024-08-28

网络出版日期: 2025-05-22

基金资助

国家自然科学基金(32302343)；贵州省烟草公司遵义市公司科技项目(2022XM09)

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Pathogenicity of Corynespora cassiicola in Different Tobacco Cultivars and Optimization of Culture Conditions

黄纯杨，杨相，周红江，史彩华，汪汉成，胡静荣

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(1. Guizhou Provincial Tobacco Company, Zunyi Branch, Zunyi 563000, Guizhou China; 2. Institute of Advanced Agricultural Science, Hubei University of Arts and Science, Xiangyang 441054, Hubei China; 3. Guizhou Academy of Tobacco Science, Guiyang 550081, Guizhou China)

Received date: 2024-07-20

Accepted date: 2024-08-28

Online published: 2025-05-22

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摘要

为明确多主棒孢霉Corynespora cassiicola的致病性及其培养条件，从烟草Nicotiana tabacum分离的多主棒孢霉菌株HBU230811接种到6个常见的烟草品种，明确其致病性并筛选其最适培养基及pH、糖源、光周期、温度等培养条件。结果表明，多主棒孢霉菌株HBU230811对6个烟草品种均有不同程度侵染，但对贵烟5号的侵染最弱。该菌株能够在PDA、MS、OA、KBA、LB和TLA等多种培养基上生长，其中KBA和OA培养基上的菌落直径无显著差异，但显著大于其他培养基的菌落。OA培养基的菌丝较稀薄，KBA培养基上的菌丝较浓密且产孢时间最短。同时，该菌株在pH 4～12范围均可正常生长，且pH为7～9时生长状态最佳。另外，糖源、光周期和温度对菌株HBU230811生长均有显著影响。其中，该菌株生长的最佳条件是以山梨醇作为糖源、28 ℃全黑暗条件培养。研究结果为揭示多主棒孢霉菌株HBU230811致病生物学特性提供了理论基础。

关键词： 烟草; 多主棒孢霉; 培养条件优化; 致病性; 生长环境

本文引用格式

HUANG Chun-yang , YANG Xiang , ZHOU Hong-jiang , SHI Cai-hua , WANG Han-cheng , HU Jing-rong . 多主棒孢霉对不同品种烟草的致病性及其培养条件优化研究[J]. 亚热带植物科学, 2025 , 54(1) : 22 -28 . DOI: 10.3969/j.issn.1009-7791.2025.01.003

Abstract

In order to elucidate the pathogenicity of Corynespora cassiicola and determine its optimization of cultivation conditions, this study conducted inoculation experiments using six common tobacco varieties inoculating with C. cassiicola strain HBU230811. The findings revealed varying levels of infection across all tested varieties; notably, Guizhou No.5 exhibited pronounced resistance against this strain. The ability for growth was observed across multiple culture media including PDA, MS, OA, KBA, LB, and TLA; although there were no discernible differences in colony diameter between KBA and OA, the size remained significantly larger compared to other media. On OA, mycelium appeared thinner whereas it displayed denser characteristics when grown on KBA, resulting in shorter spore-forming times. Moreover, the strain demonstrated normal growth within a pH range from 4 to 12, yet optimal conditions were observed at a pH level between 7 and 9. Additionally, various factors such as sugar sources, duration of light exposure, and temperature exerted significantly influences on the growth of C. cassiicola strain HBU230811. Notably, sorbitol as a sugar source combined with cultivation at 28 ℃ under complete darkness yielded the most favorable growth conditions. These findings contribute to establishing a theoretical foundation for understanding the pathogenic ecological characteristics of C. cassiicola strain HBU230811.

Key words： Nicotiana tabacum; Corynespora cassiicola; optimizing culture conditions; pathogenicity; growing environment

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