蟛蜞菊发酵液对铜绿微囊藻的化感作用

LU Xiao-gui; XIE Qin-ming

doi:10.3969/j.issn.1009-7791.2025.01.004

亚热带植物科学 >

2025 , Vol. 54 >Issue 1: 29 - 36

DOI: https://doi.org/10.3969/j.issn.1009-7791.2025.01.004

研究论文

蟛蜞菊发酵液对铜绿微囊藻的化感作用

LU Xiao-gui ,
XIE Qin-ming

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(集美大学水产学院，福建厦门361021)

收稿日期: 2024-08-12

录用日期: 2024-09-25

网络出版日期: 2025-05-22

基金资助

福建省科技计划项目农业引导性(重点)项目(2021N0013)

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Allelopathic Effect of Wedelia chinensis Fermented Broth on Microcystis aeruginosa

卢小贵，谢钦铭

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(College of Fisheries, Jimei University, Xiamen 361021, Fujian China)

Received date: 2024-08-12

Accepted date: 2024-09-25

Online published: 2025-05-22

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摘要

利用化感物质预防和控制蓝藻水华具有价格便宜、绿色可持续发展等优点，寻求高效绿色的抑藻剂至关重要。以铜绿微囊藻Microcystis aeruginosa为研究对象，探究蟛蜞菊发酵液的化感作用。结果表明，蟛蜞菊发酵液对铜绿微囊藻化感作用总体呈“低促高抑”。与对照组相比，低浓度(0.5 mg·mL^–1)发酵液对铜绿微囊藻化感作用的促进最高为17%,此时CK组细胞密度为8.73×10⁵个·mL^–1，0.5 mg·mL^–1发酵液为10.21×10⁵个·mL^–1。最高浓度(2.5 mg·mL^–1)发酵液对铜绿微囊藻化感作用抑制率最高为54%，此时CK组细胞密度为5.72×10⁵个·mL^–1，2.5 mg·mL^–1发酵液为2.61×10⁵个·mL^–1；处理10 d后铜绿微囊藻叶绿素a含量显著降低，2.5 mg·mL^–1处理组叶绿素a含量从第2天起显著低于CK组(P＜0.05），第10天时，仅为CK组叶绿素a含量的37.74%。与CK组相比，不同浓度蟛蜞菊发酵液对铜绿微囊藻3种藻蛋白的合成无显著影响(P＞0.05)。处理10 d后，蟛蜞菊发酵液对铜绿微囊藻SOD活性的影响基本随其浓度升高呈逐渐增强趋势，且2.5 mg·mL^–1发酵液处理组的铜绿微囊藻SOD活性最大，为2.25 U·mL^–1，是CK组的2.1倍。

关键词： 蟛蜞菊发酵液; 铜绿微囊藻; 化感作用

本文引用格式

LU Xiao-gui , XIE Qin-ming . 蟛蜞菊发酵液对铜绿微囊藻的化感作用[J]. 亚热带植物科学, 2025 , 54(1) : 29 -36 . DOI: 10.3969/j.issn.1009-7791.2025.01.004

Abstract

The use of allelochemicals for the prevention and control of cyanobacterial blooms is considered to have the advantages of low cost and sustainable development, seeking the efficient and eco-friendly algaecides is crucial. In this experiment, Microcystis aeruginosa was chosen as the subject to explore the allelopathic effects of Wedelia chinensis fermented broth It was found that the overall allelopathic effect of the Wedelia chinensis fermented broth on Microcystis aeruginosa exhibited a phenomenon of "low promotion and high inhibition." Compared to the control group (CK), a low concentration (0.5 mg·mL^–1) of the Wedelia chinensis fermented broth exhibited a maximum promotion effect of 17% on Microcystis aeruginosa growth, with cell densities of 8.73 × 10⁵ cells·mL^–1 in the CK group and 10.21 × 10⁵ cells·mL^–1 in the 0.5 mg·mL^–1 treatment group. In contrast, the highest concentration (2.5 mg·mL^–1) showed a maximum inhibition rate of 54%, with cell densities of 5.72 × 10⁵ cells·mL^–1 in the CK group and 2.61 × 10⁵ cells·mL^–1 in the 2.5 mg·mL^–1 treatment group. After 10 days of treatment, the chlorophyll a content in M. aeruginosa significantly decreased, with the 2.5 mg·mL^–1 experimental group showing chlorophyll levels significantly lower than those of the control group from the second day (P＜0.05), and by the tenth day, the chlorophyll content in the control group was 2.65 times that of the experimental group. Phycobiliproteins, which assist in photosynthesis, were found to be unaffected in their synthesis across different concentrations of pyrethrum Wedelia chinensis fermented broth compared to the control group (P＞0.05), the total protein of CK group and low to high concentration were 0.132, 0.134, 0.132, 0.129, 0.126, and 0.126 mg·L^–1, respectively. On the tenth day, the effect of pyrethrum Wedelia chinensis fermented broth on the SOD content in M. aeruginosa generally showed an increasing trend. The SOD content in the control group was higher than in the 0.5 mg·mL^–1 experimental group, but the difference was not significant (P＞0.05). In the experimental group with 2.5 mg·mL^–1, the SOD content peaked at 2.25 U·mL^–1, which was 2.1 times that of the control group. This study aimed to contribute foundational data for the prevention and control of cyanobacterial blooms.

Key words： Wedelia chinensis fermented broth; Microcystis aeruginosa; allelopathy

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