枫香树TCP基因家族鉴定与表达分析

ZHAO Jin-tao; YE Xing-zhuang; WANG An-bang; CHEN Zhi-yun; WENG Hui-ying; ZHANG Guo-fang

doi:10.3969/j.issn.1009-7791.2025.02.001

亚热带植物科学 >

2025 , Vol. 54 >Issue 2: 109 - 120

DOI: https://doi.org/10.3969/j.issn.1009-7791.2025.02.001

研究论文

枫香树TCP基因家族鉴定与表达分析

ZHAO Jin-tao ,
YE Xing-zhuang ,
WANG An-bang ,
CHEN Zhi-yun ,
WENG Hui-ying ,
ZHANG Guo-fang

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(1. 福建农林大学林学院，福建福州 350002；2. 福建农林大学菌草与生态学院，福建福州 350002；3. 福建省邵武卫闽国有林场，福建南平 354000)

收稿日期: 2025-01-15

录用日期: 2025-03-20

网络出版日期: 2025-06-27

基金资助

福建省林业科研项目(2023FKJ05)；福建农林大学林学学科创新团队支持计划(72202200205)

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Identification and Expression Analysis of the TCP Gene Family in Liquidambar formosana

赵金涛，叶兴状，王安邦，陈志云，翁慧莹，张国防

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(1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 2. College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 3. Weimin National Forest Farm of Shaowu, Nanping 354000, Fujian China)

Received date: 2025-01-15

Accepted date: 2025-03-20

Online published: 2025-06-27

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摘要

枫香树Liquidambar formosana是蕈树科落叶型代表树种，具有较高的经济价值和生态价值。TCP基因家族在植物生长发育及逆境适应中扮演着关键角色。基于枫香树基因组数据和转录组数据，利用生物信息学方法，在全基因组水平上鉴定LfTCP基因家族，并对其蛋白质理化性质、基因位置、系统发育进化关系、种内种间共线性关系、启动子顺式作用元件以及组织表达量进行分析。结果表明，枫香树TCP基因家族共有22个成员，编码蛋白长度介于197～552 AA之间；22个LfTCPs基因非均匀分布于12条染色体；LfTCPs在Class Ⅰ中有12个成员，在Class Ⅱ中有10个成员，其中CIN亚家族有7个成员，CYC/TB1亚家族有3个成员。LfTCP基因家族启动子顺式元件可能与光、激素和胁迫响应有关。LfTCPs基因具有组织表达特异性，LfTCP6、LfTCP7和LfTCP17在叶中特异性高表达，可作为研究枫香树叶片生长和形态建成的重要候选基因。该研究结果可为枫香树TCP家族基因的功能及调控机制提供参考。

关键词： 枫香树; TCP基因家族; 生物信息学; 组织表达

本文引用格式

ZHAO Jin-tao , YE Xing-zhuang , WANG An-bang , CHEN Zhi-yun , WENG Hui-ying , ZHANG Guo-fang . 枫香树TCP基因家族鉴定与表达分析[J]. 亚热带植物科学, 2025 , 54(2) : 109 -120 . DOI: 10.3969/j.issn.1009-7791.2025.02.001

Abstract

Liquidambar formosana, a representative deciduous species of the Altingiaceae, holds significant economic and ecological values. The TCP gene family plays a crucial role in plant growth, development, and adaptation to stress. In this study, we integrated the genomic and transcriptomic data of L. formosana and used bioinformatics approaches to identify the LfTCP gene family at the whole-genome level. We analyzed the physicochemical properties of the proteins, gene locations, phylogenetic and evolutionary relationships, intra- and interspecific collinearity, promoter cis-acting elements, and tissue-specific expression profiles. The LfTCP gene family consists of 22 members with protein lengths ranging from 197 to 552 amino acids. These 22 LfTCP genes are unevenly distributed across 12 chromosomes. Phylogenetic analysis indicates that there are 12 members in Class I and 10 members in Class II, including 7 members in the CIN subfamily and 3 members in the CYC/TB1 subfamily. The promoter cis-regulatory elements suggest that the LfTCP gene family may be involved in responses to light, hormones, and stress. Tissue-specific expression analysis shows that LfTCP genes exhibit distinct tissue expression patterns, with LfTCP6, LfTCP7, and LfTCP17 being specifically highly expressed in leaves. These genes can serve as important candidates for studying the growth and morphological development of L. formosana leaves. The results provide a reference for understanding the functions of the LfTCP gene family and the molecular mechanisms by which TCP regulates leaf growth and development in L. formosana.

Key words： Liquidambar formosana; TCP gene family; bioinformatics; tissue expression

参考文献

[1] 刘丽娟, 高辉. TCP家族基因研究进展[J]. 生物技术通报, 2016, 32: 14–22.
[2] Navaud O, Dabos P, Carnus E, Tremousaygue D, Hervé C. TCP transcription factors predate the emergence of land plants [J]. Journal of Molecular Evolution, 2007, 65: 23–33.
[3] Cubas P, Lauter N, Doebley J, Coen E. The TCP domain: a motif found in proteins regulating plant growth and development [J]. The Plant Journal: for cell and molecular biology, 1999, 18: 215–222.
[4] Martín-Trillo M, Cubas P. TCP genes: a family snapshot ten years later [J]. Trends in Plant Science, 2010, 15: 31–39.
[5] Li S. The Arabidopsis thaliana TCP transcription factors: A broadening horizon beyond development [J]. Plant Signaling & Behavior, 2015, 10: e1044192.
[6] Danisman S. TCP Transcription Factors at the Interface between environmental challenges and the plant's growth responses [J]. Frontiers in Plant Science, 2016, 7: 103389.
[7] Zhou H, Hwarari D, Ma H, Xu H, Yang L, Luo Y. Genomic survey of TCP transcription factors in plants: Phylogenomics, evolution and their biology [J]. Frontiers in Genetics, 2022, 13: 1060546.
[8] 彭晓梅, 孟晨, Garcia C P, 张宇, 杨永平, 孙旭东. 垂穗披碱草TCP转录因子家族鉴定及激素响应模式分析[J/OL]．广西植物, 2024: 1–22. Doi: 10.11931/guihaia.gxzw202402013.
[9] Wang S T, Sun X L, Hoshino Y, Yu Y, Jia B, Sun Z W, Sun M Z, Duan X B, Zhu Y M. MicroRNA319 positively regulates cold tolerance by targeting OsPCF6 and OsTCP21 in rice (Oryza sativa L.) [J]. Public Library Science, 2014, 9: e91357.
[10] Giraud E, Ng S, Carrie C, Duncan O, Low J, Lee C P, Van Aken O, Millar A H, Murcha M, Whelan J. TCP transcription factors link the regulation of genes encoding mitochondrial proteins with the circadian clock in Arabidopsis thaliana [J]. The Plant Cell, 2010, 22: 3921–3934.
[11] Diao Y, Zhan J, Zhao Y, Liu L, Liu P, Wei X, Ding Y, Sajjad M, Hu W, Wang P, Ge X. GhTIE1 regulates branching through modulating the transcriptional activity of TCPs in cotton and Arabidopsis [J]. Frontiers in Plant Science, 2019, 10: 1348.
[12] 关紫微, 曹希雅, 张先文, 周小云. 水稻TCP家族的全基因组鉴定及表达分析[J]. 分子植物育种, 2022, 20(10): 3145–3156.
[13] 丁宁, 李梓彰, 田文, 党仁美, 刘媛媛, 闻珊珊. 小麦TCP基因家族的全基因组鉴定和对热胁迫的响应[J]. 麦类作物学报, 2018, 38：1157–1165.
[14] Leng X, Wei H, Xu X, Ghuge SA, Jia D, Liu G, Wang Y, Yuan Y. Genome-wide identification and transcript analysis of TCP transcription factors in grapevine [J]. BMC Genomics, 2019, 20(1): 786.
[15] Sun R, Lin F, Huang P, Ye X, Lai J, Zheng Y. Phylogeographical Structure of Liquidambar formosana Hance Revealed by Chloroplast Phylogeography and Species Distribution Models[J]. Forests, 2019, 10(10):858.
[16] 缪建华, 曾建雄, 骆必刚, 朱小辉. 枫香树特征特性与主要播种繁殖技术探究[J]. 南方农业, 2018, 12: 30–31.
[17] Chang Y Y, Huang Y M, Chang H T. Using Headspace Gas Chromatography–Mass Spectrometry to Investigate the Volatile Terpenoids Released from the Liquidambar formosana Leaf and Its Essential Oil[J]. Forests, 2024, 15(9):1495.
[18] Wen C H, Chu F H. A R2R3-MYB gene LfMYB113 is responsible for autumn leaf coloration in formosan sweet gum (Liquidambar formosana Hance) [J]. Plant and Cell Physiology, 2017, 58: 508–521.
[19] Li Y, Zhou Y, Chen H, Chen C, Liu Z, Han C, Wu Q, Yu F. Transcriptomic analyses reveal key genes involved in pigment biosynthesis related to leaf color change of Liquidambar formosana Hance [J]. Molecules, 2022, 27(17): 2433.
[20] Yin G P, Wang Y, Xiao Y F, Yang J S, Wang R J, Jiang Y, Huang R L, Liu X S, Jiang Y. Relationships between leaf color changes, pigment levels, enzyme activity, photosynthetic fluorescence characteristics and chloroplast ultrastructure of Liquidambar formosana Hance [J]. Journal of Forestry Research, 2022, 33: 1559–1572.
[21] Li H Q, Yang B C, Sura M B, Cheng Y X, Wang F. Liquidambarines A- C, three new abietane diterpenoids from Liquidambar formosana Hance and their anti-inflammatory activities [J]. Natural Product Research, 2023, 37: 3006–3014.
[22] Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. TBtools: An integrative toolkit developed for interactive analyses of big biological data [J]. Molecular Plant, 2020, 13: 1194–1202.
[23] 张路阳, 韩文龙, 徐晓雯, 姚健, 李芳芳, 田效园, 张智强. 烟草TCP基因家族的鉴定及表达分析[J]. 生物技术通报, 2023, 39: 248–258.
[24] 杜洋, 张智韦, 陆爽, 刘雪, 李大勇, 徐朝阳, 王晨晨, 许立新, 张彬. 菊苣TCP基因家族鉴定与分析[J]. 草地学报, 2024, 32: 2428–2439.
[25] 刘璇, 陈虞超, 郭生虎, 钟楠, 石磊, 甘晓燕. 芦竹TCP家族的基因鉴定与盐胁迫下的表达分析[J]. 草业科学, 2024, 41(10): 2316–2329.
[26] 骆金婵, 靳雅荣, 杨煜琛, 祝鑫, 贾纪原, 王小珊, 黄琳凯. 鸭茅TCP基因家族成员鉴定及表达分析[J]. 草地学报, 2024, 32: 1682–1692.
[27] Kosugi S, Ohashi Y. DNA binding and dimerization specificity and potential targets for the TCP protein family [J]. Plant Journal, 2002, 30: 337–348.
[28] 杨兰锋, 朱旭东, 周宾寒, 罗旖柔, 李明辉, 方正武. 苦荞TCP转录因子全基因组鉴定及非生物胁迫分析[J]. 广西植物, 2024, 44(07): 1364-1376.
[29] Chai W, Jiang P, Huang G, Jiang H, Li X. Identification and expression profiling analysis of TCP family genes involved in growth and development in maize [J]. Physiology and Molecular Biology of Plants, 2017, 23: 779–791.
[30] 张宝月, 刘国平, 田金红, 王大玮. 云南栘??TCP基因家族全基因组鉴定与表达分析[J]. 生物工程学报, 2025, 41(2): 809–824.
[31] Tian J H, Shu J X, Xu L, Ya H, Yang L, Li S G, Wang D W. Genome-wide identification of R2R3-MYB transcription factor family in Docynia delavayi (Franch.) Schneid and its expression analysis during the fruit development [J]. Food Bioscience, 2023, 54: 102878.
[32] Nicolas M, Cubas P. TCP factors: new kids on the signaling block [J]. Current Opinion in Plant Biology, 2016, 33: 33–41.
[33] Doebley J, Stec A, Hubbard L. The evolution of apical dominance in maize [J]. Nature, 1997, 386: 485–488.
[34] Finlayson S A. Arabidopsis TEOSINTE BRANCHED1–LIKE 1 regulates axillary bud outgrowth and is homologous to monocot TEOSINTE BRANCHED1 [J]. Plant and Cell Physiology, 2007, 48: 667–677.
[35] Efroni I, Blum E, Goldshmidt A, Eshed Y. A protracted and dynamic maturation schedule underlies Arabidopsis leaf development [J]. The Plant cell, 2008, 20: 2293–2306.
[36] Nath U, Crawford BC, Carpenter R, Coen E. Genetic control of surface curvature [J]. Science, 2003, 299: 1404–1407.
[37] Crawford B C, Nath U, Carpenter R, Coen E S. CINCINNATA controls both cell differentiation and growth in petal lobes and leaves of antirrhinum [J]. Plant Physiology, 2004, 135: 244–253.
[38] Li Y, Wang R X, Yu L X, Yu H Y, Zhu Y W, Zhao Y K, Liu Y R, Tang Y S, Zhu Y M, Han R, Pei X N, Zhao X Y. Unraveling a growth-promoting potential for plants: Genome-wide identification and expression state of the TCP gene family in Juglans mandshurica [J]. Scientia Horticulturae, 2025. DOI: 10.1016/j.scienta.2024.113852.

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