锥栗贮藏期间主要致病菌的分离及鉴定

李辉耀, 林淑婷, 范紫薇, 马春华, 傅新征, 陈艺晖, 张 静

doi:10.3969/j.issn.1009-7791.2026.01.007

亚热带植物科学 >

2026 , Vol. 55 >Issue 1: 64 - 72

DOI: https://doi.org/10.3969/j.issn.1009-7791.2026.01.007

研究论文

锥栗贮藏期间主要致病菌的分离及鉴定

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(1. 福建农林大学食品科学学院，福建福州 350002；2. 武夷学院茶与食品学院，福建武夷山 354300；3. 亚热带特色农产品采后生物学福建省高校重点实验室，福建福州 350002)

收稿日期: 2025-10-15

录用日期: 2026-01-06

网络出版日期: 2026-02-28

基金资助

福建省科技计划项目（2020N0036）；福建省中青年教师教育科研项目（科技类）（JAT210455）

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Isolation and Identification of Major Pathogenic Fungi During Castanea henryi Storage

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(1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 2. College of Tea and Food Sciences, Wuyi University, Wuyishan 354300, Fujian China; 3. Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Province University, Fuzhou 350002, Fujian China)

Received date: 2025-10-15

Accepted date: 2026-01-06

Online published: 2026-02-28

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摘要

锥栗在贮藏期间易发生霉烂和腐败，主要原因是病原菌侵染。本研究以闽北主要栽培品种油榛锥栗为材料，从腐败栗肉组织中分离、纯化病原菌，结合形态学与分子生物学方法进行鉴定，构建系统发育树，以明确分离菌株的分类地位。结果表明，共鉴定出的致病菌株包括 Gd21Ⅱ、Dd13、Dc21、Ga11、Gd21Ⅰ、Bc21、Gd12Ⅱ、Fd12Ⅱ、Ad11、Fd12Ⅰ、Fd13、Dd11 和 Gb23，分别归属于青霉属Penicillium mallochii、枝孢属Cladosporium sp.、葡萄座腔菌Botryosphaeria dothidea、德巴利氏酵母属Debaryomyces robertsiae、假丝酵母属Candida sp.、间座壳属Diaporthe sp.和假单胞菌属Pseudomonas oryzihabitans。本研究通过对锥栗贮藏期间主要病原菌的分离与鉴定，明确了闽北锥栗致病菌的种类及其系统分类，为锥栗采后贮藏保鲜技术的目标致病微生物提供了标靶目标，并为锥栗保鲜新技术开发提供了理论依据。

关键词： 锥栗; 分离纯化; 致病菌; 鉴定

本文引用格式

李辉耀, 林淑婷, 范紫薇, 马春华, 傅新征, 陈艺晖, 张静 . 锥栗贮藏期间主要致病菌的分离及鉴定[J]. 亚热带植物科学, 2026 , 55(1) : 64 -72 . DOI: 10.3969/j.issn.1009-7791.2026.01.007

Abstract

Castanea henryi is susceptible to spoilage during storage, primarily due to pathogenic infection. In this study, the main cultivated variety C. henryi 'Youzhen' in northern Fujian were used as experimental material. Pathogenic fungi were isolated and purified from decayed kernel tissues, followed by identification based on morphological and molecular biological methods. A phylogenetic tree was constructed to clarify the taxonomic status of the isolated strains. The results showed that the pathogens responsible for C. henryi spoilage included Gd21Ⅱ, Dd13, Dc21, Ga11, Gd21Ⅰ, Bc21, Gd12Ⅱ, Fd12Ⅱ, Ad11, Fd12Ⅰ，Fd13, Dd11, and Gb23, which were respectively classified as Penicillium mallochii, Cladosporium sp., Botryosphaeria dothidea, Debaryomyces robertsiae, Candida sp., Diaporthe sp., and Pseudomonas oryzihabitans. Through the isolation and identification of major pathogenic fungi during storage, this study clarifies the species and phylogenetic classification of the spoilage-causing microorganisms, providing a theoretical basis for the development of postharvest storage and preservation techniques for C. henryi. It also provides a theoretical basis for the development of new preservation technologies for C. henryi.

Key words： Castanea henryi; isolation and purification; pathogen; identification

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