Chemical mutation was an effective way of creating new germplasm resources. To analyze the impact of chemical mutagenesis on AS-9 maize inbred offspring, the salt tolerance and genetic variation between mutant M4 and the corresponding original material (AS-9) were evaluated by using 44 pairs of SSR markers that may be associated with root traits. Seed germination, bud length and root number of M4 were higher than that of AS-9. After treatment with 200 and 250 mmol.L-1 NaCl for 7 days, root numbers of IV400 and IV1600 seedlings were higher than that of AS-9, with increases of 23.1% and 19.1%, respectively.IV1600 primary root lengths of 1, 2, 3 were 1.33, 1.70, and 2.15 times longer than that of AS-9, respectively. Twenty five pairs of SSR primers gave profiles amplified in sample of M4 mutants, accounting for 56.82% of the total pairs of primers. The average value of genetic similarity coefficient between IVCK and M4 was 0.6563. With the centered genetic similarity coefficient of -0.04 by UPGMA, the maize inbred line materials were divided into three groups: the original material (IVCK), the M4 mutants including IV1400, IV1600, IV2000, and other M4 mutants (IV200, IV400, IV800, IV1200). Principal component analysis and clustering results were similar. In addition, SSR-PCR products amplified by six pairs of primers were sequenced, the results showed that there was distinct variation between AS-9 and the induced M4, indicating chemical mutagenesis made maize inbred AS-9 produce a wide range of genetic variation.
[1] 徐明,路铁刚. 植物诱变技术的研究进展[J]. 生物技术进展, 2011,1(2): 90—97.
[2] 魏良明,姜鸿勋,胡学安. 植物诱变新技术及其在玉米育种上的应用[J]. 玉米科学, 2000,8(1): 19—20.
[3] 崔霞,梁燕,李翠,秦蕾,李云洲. 化学诱变及其在蔬菜育种中的应用[J]. 西北农林科技大学学报(自然科学版), 2013,41(3): 1—8.
[4] 覃鸿妮,蔡一林,杨春蓉,王国强. 玉米诱变系的SSR遗传变异分析[J]. 核农学报, 2008,22(6): 750—755.
[5] Inukai Y, Sakamoto T, Ueguchi-Tanaka M, Shibata Y, Gomi K, Umemura I, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M. Crown rootless 1,which is essential for crown root formation in rice, is a target of an auxin response factor in auxin signaling[J]. Plant Cell, 2005,17: 1387—1396．
[6] 朱保葛,路子显,耿玉轩,邓向东,谷爱秋. 烷化剂EMS诱发花生性状变异的效果及高产突变系的选育[J]. 中国农业科学, 1997,30(6): 87—89.
[7] Braaten D C, Thomas J R, Little R D, Dickson K R, Goldberg I, Schlessinger D, Ciccodicola A, D Urso M. Locations and contexts of sequences that hybridize to poly(dG-dT)?(dC-dA) in mammalian ribosomal DNAs and two X-linked genes[J]. Nucleic Acids Research,1998,16(3): 865—881.
[8] 尹冬冬,安调过,李立会,许红星. 分子标记技术在黑麦研究中的应用[J]. 中国生态农业学报, 2011,19(2): 477—483.
[9] Wang F G, Tian H L, Zhao J R, Yi H M, Wang L, Song W. Development and characterization of a core set of SSR markers for fingerprinting analysis of Chinese maize varieties [DB/OL]. Maydica, 2011,56: 1693.
[10] 刘志斋,吴迅,刘海利,李永祥,李清超,王凤格,石云素,宋燕春,宋伟彬, 赵久然, 赖锦盛,黎裕,王天. 基于40个核心SSR标记揭示的820份中国玉米重要自交系的遗传多样性与群体结构[J]. 中国农业科学, 2012,45(11): 2107—2138.
[11] 张雁明,邢国芳,刘美桃,刘晓东,韩渊怀. 全基因组关联分析: 基因组学研究的机遇与挑战[J]. 生物技术通报, 2013(6): 1—5.
[12] 王倩,毛新国,昌小平,贾继增,刘惠民,景蕊莲. 小麦TaSnRK2.10的多态性及与农艺性状的关联[J]. 中国农业科学 2014,47(10): 1865—1877.
[13] Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants: Present status and future prospects[J]. Plant Molecular Biology, 2005,57: 461—485.
[14] Zondervan K T, Cardon L R. The complex interplay among factors that influence allelic association[J]. Nature Reviews Genetics, 2004,5(2): 89—100.
[15] 赵曦,王荣焕,于永涛,王天宇,黎裕. 关联分析在玉米遗传学研究中的应用[J]. 玉米科学, 2008,16(1): 52—55.
[16] Agrama H A, Eizenga G C, Yan W. Association mapping of yield and its components in rice cultivars[J]. Molecular Breeding, 2007,19: 341—356.
[17] Eizenga G C, Agrama H A, Lee F N, Yan W, Jia Y. Identifying novel resistance genes in newly introduced blast resistant rice germplasm[J]. Crop Science, 2006,46: 1870—1878.
[18] Tuberosa R,Sanguineti M C, Landi P. Identification of QTLs for root characteristics in maize grown in hydroponics and analysis of their overlap with QTLs for grain yield in the field at two water regimes[J]. Plant Molecular Biology, 2002,48: 697—712.
[19] 张微. 利用RIL群体定位玉米苗期根系QTL的研究[D]. 北京: 中国农业大学硕士学位论文, 2005.
[20] 高世斌,冯质雷,李晚忱,荣廷昭. 干旱胁迫下玉米根系性状和产量的QTLs分析[J]. 作物学报, 2005,31(6): 718—722.
[21] 李红英,卢存福,兰小中,陈玉珍. 玉米自交系AS-9化学诱变后代SSR遗传变异分析[J]. 华北农学报, 2013,28(3): 92—101.
[22] 乔晓,石海春,柯永培. 玉米航天诱变 SP3 株系的遗传变异分析[J]. 玉米科学, 2012,20(3): 15—21.
[23] 李奇,石海春. 玉米自交系48-2和R08辐照后代M3株系遗传变异的SSR分析[J]. 核农学报, 2011,25(6): 1100—1106.
[24] 秦家友,石海春,柯永培,余学杰,袁继超. 玉米辐射诱变系表型及SSR遗传差异研究[J]. 玉米科学, 2012,20(2): 41—47.
[25] Kostova A, Todorovska E, Christova N, Hristov K, Atanassov A. Assessment of genetic variability induced by chemical mutagenesis in elite maize germplasm via SSR markers[J]. Crop Improvement, 2006,16( 1—2): 37—48．
[26] 杨小红,严建兵,郑艳萍,余建明,李建生. 植物数量性状关联分析研究进展[J]. 作物学报, 2007,33(4): 523—530.
[27] 赵振卿,顾宏辉,盛小光,虞慧芳,王建升,曹家树. 作物数量性状位点研究进展及其育种应用[J]. 核农学报, 2014,28(9): 1615—1624.
闽公网安备 35020602000789号