植物生理生化与分子生物学

水稻矮杆多分蘖突变体CA648的光合生理特性分析

  • 刘晓鹏 ,
  • 朱允华 ,
  • 刘 清 ,
  • 史 齐 ,
  • 李合松
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  • (1.湖南农业大学 植物激素与生长发育湖南省重点实验室,湖南 长沙 410128;2.南华大学 药学与生物科学学院,湖南 衡阳 421001)
刘晓鹏,硕士研究生,从事分子生物物理研究。

收稿日期: 2015-01-09

  修回日期: 2015-04-18

  网络出版日期: 2015-03-10

基金资助

国家科技支撑计划(2011BAD16B01);湖南省高校创新平台开放基金项目(09k052,13k065);作物种质创新与资源利用重点实验室开放研究项目(12KFXM05)

Photosynthetic Physiological Analysis on Dwarf Multi-Tillering Rice Mutant CA648

  • LIU Xiao-peng ,
  • ZHU Yun-hua ,
  • LIU Qing ,
  • SHI Qi ,
  • LI He-song
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  • (1.Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, Hunan China; 2.College of Pharmacy and Biological Sciences, Nanhua University, Hengyang 421001, Hunan China)

Received date: 2015-01-09

  Revised date: 2015-04-18

  Online published: 2015-03-10

摘要

本研究分析了水稻矮杆多分蘖突变体丛矮648 (CA648)及对照中花11 (ZH11)在分蘖期叶片的叶绿素含量、光合速率,还原糖、蔗糖含量以及POD酶活力。结果表明,分蘖期ZH11剑叶中叶绿素含量高于CA648。水稻分蘖前期,CA648的净光合速率、气孔导度、蒸腾速率都低于ZH11,胞间CO2浓度高于ZH11;而在分蘖盛期(分蘖期后20 d)两种材料的净光合速率差异显著,ZH11净光合速率比CA648高17.5%,同时CA648的气孔导度、胞间CO2浓度和蒸腾速率都低于ZH11;而分蘖后期ZH11与CA648净光合速率几乎相同。分蘖盛期CA648叶片还原糖含量明显比对照高;而茎杆还原糖含量与对照无差异。CA648叶片蔗糖含量低于对照,约为ZH11的77.5%;而茎杆的蔗糖含量高于对照,比ZH11高30.7%。两种材料POD酶活力差异明显,其中在分蘖盛期,CA648叶、茎中的POD酶活力分别比ZH11高73.0%、16.8%。

本文引用格式

刘晓鹏 , 朱允华 , 刘 清 , 史 齐 , 李合松 . 水稻矮杆多分蘖突变体CA648的光合生理特性分析[J]. 亚热带植物科学, 2015 , 44(01) : 18 -22 . DOI: 10.3969/j.issn.1009-7791.2015.01.004

Abstract

The chlorophyll content, photosynthetic rate, sugar and sucrose contents, POD enzyme activity of rice mutant (CA648, performing a dwarf and multi-tillering phenotype) and wild type ZH11 were preliminary studied. The results showed that the chlorophyll content of ZH11 was higher than that of CA648 at tillering stage. At the early stage of tillering, the net photosynthetic rate, stomatal conductance, and transpiration rate of CA648 were lower than those of ZH11, but intercellular CO2 concentration in CA648 was higher than that in ZH11. At the top tillering stage (20 d after tillering stage), the significant difference of the net photosynthetic rates of two kinds of material was found. The net photosynthetic rate of ZH11 was about 17.5% higher than CA648. Meanwhile, stomatal conductance, transpiration rate and intercellular CO2 concentration of ZH11 were also higher than those of CA648. At the late tillering stage, the net photosynthetic rates of ZH11 and CA648 were almost the same. At the top tillering stage, reducing sugar content in CA648 leaf was significantly higher than the control, while there was no difference of reducing sugar content in the two kinds of stems. The sucrose content of leaf in CA648 was lower than the control, about 77.5% of ZH11, while the sucrose content in the stem was 30.7% higher than ZH11. There were obvious differences of POD enzyme activity between two kinds of material. At the mid-tillering stage, the POD activities in leaf and stem of CA648 were 73.0% and 16.8% higher than those in ZH11, respectively.

参考文献

[1] 虞国平. 水稻在我国粮食安全中的战略地位分析[J]. 新西部(理论版), 2009(11): 31—33.

[2] 文杜娟,陈风波. 杂交水稻种植对社会经济的影响[J]. 作物研究, 2014(4): 402—405.

[3] 程式华. 中国超级稻育种研究的创新与发展[J]. 沈阳农业大学学报, 2007(5): 647—651.

[4] 冯荣坤. 超高产水稻育种中理想株型研究进展[J]. 河南农业科学, 2006(7): 19—22.

[5] 唐茜,叶善蓉,单洪丽,罗学平. 引进茶树品种光合特性的比较研究[J]. 四川农业大学学报, 2006,24(3): 303—308.

[6] 乔亮. 水稻蔗糖合成酶基因家族的初步研究[D]. 济南: 山东大学硕士学位论文, 2012.

[7] 黄雪方,金雅琴,李冬林. 两种石蒜生长发育期鳞茎可溶性糖、蛋白质及POD活性的变化[J]. 亚热带植物科学, 2011(3): 16—19.

[8] 刘清,童建华,史齐,彭克勤,王若仲,蔺万煌, Mohammed H K,沈革志,萧浪涛. 一个矮秆多分蘖水稻突变体的植物激素动态特性分析[J]. 中国农业科学, 2014(13): 2519—2528.

[9] 王惠群,肖浪涛,杨艳丽,童建华,康朵兰. 矮壮素对中薯3号生理特性的影响研究[J]. 云南农业大学学报, 2008,23(2): 200—204.

[10] 郝再彬,苍晶,徐仲. 植物生理实验[M]. 哈尔滨: 哈尔滨工业大学出版社, 2004.

[11] 张迟. 抑制马铃薯Acid Invertase基因表达对块茎淀粉—糖代谢的影响研究[D]. 武汉: 华中农业大学博士学位论文, 2007.

[12] 张志良. 植物生理实验指导手册[M]. 北京: 高等教育出版社, 2000.

[13] 陈庭木,王多明,方兆伟,秦德荣,樊继伟,吕孝财,徐大勇. 水稻超高产潜力鉴定重要农艺性状因子分析[J]. 安徽农业科学, 2013(16): 7081—7085.

[14] 许大全. 光合作用气孔限制分析中的一些问题[J]. 植物生理学通讯, 1997,33(4): 241—244.

[15] Intapruk C, Yamamoto K, Sekine M Takano M, Shinmyo A. Regulatory sequences involved in the peroxidase gene expression in Arabidopsis thaliana[J]. Plant Cell Reports, 1994,13: 123—129.

[16] 李春俭. 植物激素在顶端优势中的作用[J]. 植物生理学通讯, 1995(6): 401—406.

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