以四数九里香新生带腋芽茎段为外植体，研究取材时期、6-BA浓度及生长素种类对外植体生长的影响，探讨不同浓度活性炭对外植体褐化的影响。结果表明，四数九里香组织培养适宜的取材时期为4月；外植体腋芽诱导率在6-BA浓度为2.5 mg·L-1时表现较高；IBA是较适宜外植体生长的生长素；活性炭浓度为1.5 g·L-1可有效减轻外植体褐化；组织培养适宜的启动培养基为MS + 6-BA 2.5 mg·L-1 + IBA 0.1 mg·L-1 + GA3 0.3 mg·L-1 + 活性炭1.5 g·L-1。
Stems with newborn axillary bud used as explant, sampling times, concentrations of 6-BA, type of auxins were researched as important factor affecting growth of explants. Also, the effect of activated carbon on browning of explants was studied. Appropriate sampling time for tissue culture of Murraya tetramera was April, appropriate 6-BA concentration for the shoots induction from explants was 2.5 mg·L-1, appropriate auxin was IBA and 1.5 g·L-1 activated carbon could reduce the browning of explants. The results showed that the initial media was MS + 6-BA 2.5 mg·L-1 + IBA 0.1 mg·L-1 + GA3 0.3 mg·L-1 + AC 1.5 g·L-1 in tissue culture of M. tetramera.
[1] Neeraj S, Natarajan S, Rao C N R. A novel open-framework zinc phosphate with intersecting helical channels[J]. Chemical Communications, 1999(2): 165—166.
[2] Garc??a-Serrano L A, Rey F, Pérez-Pariente J, Sastre E. Thermal analysis of large pore microporous zincophosphates[J]. Thermochimica Acta, 2001,376(2): 155—162.
[3] 全国中草药汇编编写组. 全国中草药汇编(上册)(第2版)[M]. 北京: 人民卫生出版社, 1996: 10.
[4] 王港,李周岐,刘晓敏,侯娜,刘淑明. 花椒组织培养再生体系的建立[J]. 西北林学院学报, 2008,23(3): 117—119.
[5] 黄靖. 金桔无性快繁体系建立的研究[J]. 江西农业学报, 2010,22(7): 60—62.
[6] 徐涌,孙骏威,陈珍. 不同植物生长调节物质处理对吴茱萸组织培养的影响[J]. 浙江农林大学学报, 2011,28(3): 500—504.
[7] 唐征,荆赞革,张小玲,罗天宽,刘庆,朱世扬. 吴茱萸组织培养和快速繁育技术研究[J]. 安徽农业科学, 2012,40(36): 17475—17477.
[8] 时群. 两面针组培技术试验[J]. 林业科技开发, 2013,27(6): 117—120.
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