红果参果实多糖的提取及其单糖组分研究

李 斌; 李 贵; 陈功锡; 李 辉; 梁俊鹏; 陈 雅

doi:10.3969/j.issn.1009-7791.2015.01.003

亚热带植物科学 >

2015 , Vol. 44 >Issue 01: 13 - 17

DOI: https://doi.org/10.3969/j.issn.1009-7791.2015.01.003

植物生理生化与分子生物学

红果参果实多糖的提取及其单糖组分研究

李斌 ,
李贵 ,
陈功锡 ,
李辉 ,
梁俊鹏 ,
陈雅

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(1.吉首大学植物资源保护与利用湖南省高校重点实验室，湖南吉首 416000；2.中国药科大学，江苏南京 210000；3.吉首大学生物资源与环境科学学院，湖南吉首 416000)

网络出版日期: 2015-03-10

基金资助

湖南省教育厅重点项目(09A073)

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Extraction and Composition of Polysaccharide from the Fruits of Campnumoea lancifolia

LI Bin ,
LI Gui ,
CHEN Gong-xi ,
LI Hui ,
LIANG Jun-peng ,
CHEN Ya

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(1.Key Laboratory of Plant Resource Conservation and Utilization, College of Hunan Province, Jishou University, Jishou 416000, Hunan China; 2.China Pharmaceutical University, Nanjing 210000, Jiangsu China; 3.College of Biology and Environmental Sciences, Jishou University, Jishou 416000, Hunan China)

Online published: 2015-03-10

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摘要

用超声波法提取红果参果实的多糖，并分别以乙醇提取，乙酸乙酯、sevage试剂等进行纯化，以苯酚-硫酸法测定多糖含量，以GC-MS测定其单糖组分。结果表明，红果参果实中多糖含量高达45.80%，其主要单糖组分及百分比为阿拉伯糖(5.00%)、木糖(9.65%)、甘露糖(11.55%)、果糖(30.20%)、半乳糖(33.85%)。

关键词： 红果参; 多糖; 超声提取; 单糖组成; GC-MS; 红果参; 多糖; 超声提取; 单糖组成; GC-MS; 红果参; 多糖; 超声提取; 单糖组成; GC-MS

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李斌 , 李贵 , 陈功锡 , 李辉 , 梁俊鹏 , 陈雅 . 红果参果实多糖的提取及其单糖组分研究[J]. 亚热带植物科学, 2015 , 44(01) : 13 -17 . DOI: 10.3969/j.issn.1009-7791.2015.01.003

Abstract

Polysaccharides (PS) from the fruits of Campnumoea lancifolia were extracted and purified by ethanol precipitation, ethyl acetate and sevage reagent with ultrasonic assistance, and the content was determined by the phenol-sulfuric acid method. The components of the polysaccharides were identified by gas chromatography-mass spectrometry (GC-MS). The results showed that the average content of polysaccharide in the fruits of C. lancifolia reached up to 45.80%, and main monosaccharide composition and relative contents were arabia sugar (5.00%), xylose (9.65%), mannose (11.55%), fructose (30.20%), galactose (33.85%).

Key words： Campnumoea lancifolia; polysaccharide; ultrasonic extraction; monosaccharide composition; GC-MS; Campnumoea lancifolia; polysaccharide; ultrasonic extraction; monosaccharide composition; GC-MS

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