对番石榴Psidium guajava叶乙醇提取物中熊果酸富集工艺的除杂和酸碱富集主要环节进行优化试验。结果表明，最佳富集工艺为：以料液比为1∶50的水和石油醚除去杂质；酸碱富集过程中富集条件以10% NaOH碱化提取液pH=14，再以5% H2SO4酸化调节pH=3，用1倍样品溶液体积的pH=3酸性水溶液稀释。终产品中熊果酸的纯度和回收率分别为17.74%和85.12%。
The enrichment technologies of ursolic acid in ethanol extract from Psidium guajava leaves were carried out. The results showed that the impurities were separated better with the solid-liquid ratio of 1∶50 of water and 1∶50 of petroleum ether. The conditions of accumulating ursolic acid with acid and alkali were followed as: the solution was treated with 10% NaOH to pH=14, then with 5% H2SO4 to pH=3, diluted with aqueous solution with pH=3 as the same volume, the purity and recovery of ursolic acid in the end product were 17.74% and 85.12% respectively.
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