应用系统相关分析法和灰色关联度分析法,研究伊贝母(新疆贝母Fritillaria walujewii、伊犁贝母F. pallidiflora)有效成分含量与气候因子的相关性,筛选影响其质量的主导因子。结果表明,新疆不同产地气候因子存在较大差异,伊贝母总生物碱和西贝母碱含量与年均气温、地面温度、日照时数、相对湿度、降水量等5个气候因子无显著相关性;西贝母碱苷含量与年均相对湿度有极显著正相关(P<0.01),与年均地面温度有显著正相关(P<0.05),与年均日照时数有显著负相关(P<0.05);水溶性成分腺苷含量与年均降水量和地面温度均有显著正相关(P<0.05);水溶性成分β-胸苷含量与年均气温、相对湿度和地面温度均有显著正相关(P<0.05),与年均日照时数有显著负相关(P<0.05)。上述相关分析结果与灰色关联度分析结果一致。年均气温、降水量、相对湿度和地面温度升高有助于伊贝母中西贝母碱苷、腺苷和β-胸苷的形成与积累,年均日照时数升高则抑制西贝母碱苷和β-胸苷的形成与积累。伊贝母最佳适宜生长的气候条件为年均气温13~27℃,降水量169~288 mm,相对湿度55%~66%,日照时数2140~2547 h,地面温度26~41 ℃。
The correlation between climate factors and contents of ingredient of bulbus fritillariae pallidiflorae was studied by method of correlation analysis and gray correlation analysis, and the leading affecting factors were also screened out. The results showed that the climatic factors varied greatly in different places of Xinjiang. The content of alkaloids and sipeimine have no significant correlation with average annual temperature, ground temperature, sunshine hours, relative humidity, rainfall. The content of sipeimine-3β-D-glucoside have significant positive correlation with relative humidity (P<0.01) and ground temperature (P<0.05) but have significant negative correlation with sunshine hours(P<0.05). The content of adenosine have significant positive correlation with rainfall and ground temperature (P<0.05). The content of thymidine have significant positive correlation with average annual temperature, ground temperature, relative humidity (P<0.05) but have significant negative correlation with sunshine hours(P<0.05). This result was consistent with the gray correlation analysis results. The best climate conditions for average temperature was 13—27℃, precipitation was 169—288 mm, sunshine hours was 2140—2547 h, the ground temperature was 26—41 ℃. The average temperature, precipitation, relative humidity, ground temperature played a role in the promoting synthesis and metabolism of ingredient, but the sunshine hours was inhibition synthesis and metabolism of ingredient of bulbus fritillariae pallidiflorae. The best climate of bulbus fritillariae pallidiflorae to select its GAP cultivation base, this has best significance for synthesis and accumulation of the major active ingredient of bulbus fritillariae pallidiflorae.
[1] 国家药典委员会编. 中国药典(一部)[M]. 北京: 化学工业出版社, 2010: 132.
[2] 中国植物志编辑委员会. 中国植物志(第14卷)[M]. 北京: 科学出版社, 1980: 102.
[3] 新疆生物土壤沙漠研究所. 新疆药用植物志[M]. 乌鲁木齐: 新疆人民出版社, 1977: 186 —199.
[4] 王立春. 气象要素与马铃薯产量、品质关系的研究[J]. 试验研究, 2008,7: 79—81.
[5] 李萍,季晖,徐国钧,徐珞珊. 贝母类中药的镇咳祛痰作用研究[J]. 中国药科大学学报, 1993,24(6): 360—362.
[6] Kang D G, Sohn E J, Lee Y M, Lee A S, Han J H, Kim T Y, Lee H S. Effects of bulbus Fritillaria water extract on blood pressure and renal function in the L-NAME-induced hypertensive rats[J]. Journal of Ethnopharmacology, 2004,91(1): 51—53.
[7] 黄丽晶,高文远,李霞,张艳军. 平贝母水提物抗炎作用研究[J]. 天津中医药, 2009,26(6): 495—496.
[8] 邹冈. 基础神经药理学[M]. 北京: 科学出版社, 1988.
[9] Oloumi H, Hassibi N. Study the correlation between some climate parameters and the content of phenolic compounds in roots of Glycyrrhiza glabra[J]. Journal of Medicinal Plants Research, 2011,25(5): 6011—6016.
[10] 冯旭芹,崔秀明,陈中坚,张义玲,张文生. 三七有效成分与气候生态因子的相关性分析[J]. 中国农业气象, 2006,27(1): 16.
[11] 安露莎. 野生与栽培伊贝母质量评价研究[M]. 乌鲁木齐: 新疆医科大学, 2014.
[12] 林海明,张文霖. 主成分分析与因子分析的异同和SPSS软件[J]. 统计研究, 2005(3): 65—69.
[13] 刘明芝,周仁郁. 中医药统计学与软件应用[M]. 北京: 中国中医药出版社, 2006: 280—284.
[14] 李永健,方肇勤. 聚类分析在中医药研究中的应用与思考[J]. 南京中医药大学学报(自然科学版), 2001,17(3): 182—185.
[15] 蔡萍,刘才英,梁雪娟,肖娟,万丹,张水寒. 杜仲药材有效成分与环境因子的灰色关联度分析[J]. 中国实验方剂学, 2014, 20(23): 10—14.
[16] 孙芳芳. 浅议灰色关联度分析方法及其应用[J]. 科技信息, 2010,27(17): 364—366.
[17] 盛萍,安露莎,苗莉娟,姚蓝,史红,张煊. 不同产地野生与栽培伊贝母药材水溶性成分指纹图谱研究[J]. 中国野生植物资源, 2014,33(4): 19—24.
[18] 范卓文,雷捷. 日照强度对贝母总生物碱含量的影响[J]. 哈尔滨师范大学自然科学学报, 2003,19(2): 98—99.
[19] 谢炳胜. 贝母的栽培技术[J]. 农技服务, 2014,31(11): 40—41.