研究报告

病毒唑对大花蕙兰CyMV及ORSV脱毒效果初探

  • 许传俊 ,
  • 黄珺梅 ,
  • 曾碧玉 ,
  • 张文惠 ,
  • 许文江
展开
  • 福建省亚热带植物研究所 福建省亚热带植物生理生化重点公共实验室,福建 厦门 361006
许传俊(1970-),女,安徽固镇人,副研究员,博士,从事植物细胞工程及分子生物学研究。

收稿日期: 2011-08-26

  修回日期: 2011-08-26

  网络出版日期: 2012-03-10

基金资助

福建省省属公益类研究院所基本科研专项(2009R10005-1)

Influence of Ribavirin on Cymbidium hybridum Virus Eradication

  • XU Chuan-jun ,
  • HUANG Jun-mei ,
  • ZENG Bi-yu ,
  • ZHANG Wen-hui ,
  • XU Wen-jiang
Expand
  • Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China

Received date: 2011-08-26

  Revised date: 2011-08-26

  Online published: 2012-03-10

摘要

在大花蕙兰茎尖培养中结合病毒唑处理,探讨对建兰花叶病毒(CyMV)和齿兰环斑病毒(ORSV)的脱除效果。结果表明,病毒唑添加到培养基中,处理茎尖2周,脱毒效果低于经病毒唑处理3个月后的幼苗,经病毒唑处理幼苗再结合茎尖培养脱毒效果最好。病毒唑处理对大花蕙兰CyMV的脱除效果优于对ORSV的脱除效果。

本文引用格式

许传俊 , 黄珺梅 , 曾碧玉 , 张文惠 , 许文江 . 病毒唑对大花蕙兰CyMV及ORSV脱毒效果初探[J]. 亚热带植物科学, 2012 , 41(01) : 7 -9 . DOI: 10.3969/j.issn.1009-7791.2012.01.002

Abstract

The optimum approach for producing the ORSV-free and CyMV-free Cymbidium hybridum by using three virus deprivation methods was discussed in this research, which were ribavirin treatments combining with shoot tips culture or seedlings and shoot tips came from seedlings had been treated by ribavirin for three months. The results showed that the ribavirin added to the medium treat shoot tips for two weeks was not effective against CyMV and ORSV. Shoot tips came from seedling which were treated by ribavirin was the best method, and the method of ribavirin treatment seedling for three months came second. Ribavirin was more effective against CyMV than ORSV in Cymbidium hybridum.

参考文献

[1] Zettler F W, et al. Viruses of orchids and their control[J]. Plant Disease, 1990,74: 621-626.

[2] 葛胜娟.植物组织培养中的快繁与脱毒技术及其应用[J]. 农业生物技术科学, 2005,21(5): 104-107.

[3] 董伟清,等.玉林大蒜茎尖脱毒与繁殖技术研究[J].广西农业科学, 2009,40(10): 1 289-1 291.

[4] 孙琦,等.植物脱毒与检测研究进展[J]. 山东农业大学学报(自然科学版), 2003,34(2): 307-310.

[5] 姜玲,等. 墨兰组织培养结合化学处理脱除建兰花叶病毒(CymMv)的研究[J]. 园艺学报, 2005,32(6): 1 056-1 060.

[6] Sharma S, et a1. Production of Indian citrus ringspot virus free plants of kinnow employing chemotherapy coupled with shot tip grafting[J]. Journal of Central European Agriculture, 2007,8(1): l-8.

[7] Paunovic S, et a1. In vitro production of plum pox virus-free plums by chemotherapy with ribavirin[J]. Biotechnol & Biotechnol EQ, 2007,4: 417-421.

[8] Danci O, et a1. Influence of ribavirin on potato plants regeneration and virus eradication[J]. J of Horticulture, Forestry and Biotechnology, 2009,12: 42l-425.

文章导航

/

〈 〉