芥蓝组织培养研究

陈琼娥; 钟凤林; 林碧英

doi:10.3969/j.issn.1009-7791.2011.01.009

亚热带植物科学 >

2011 , Vol. 40 >Issue 01: 34 - 37

DOI: https://doi.org/10.3969/j.issn.1009-7791.2011.01.009

研究报告

芥蓝组织培养研究

陈琼娥 ,
钟凤林 ,
林碧英

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1. 福建农林大学园艺学院, 福建福州 350002;
2. 福建农林大学蔬菜研究所, 福建福州 350002

陈琼娥(1986-),女,福建泉州人,硕士研究生,从事园艺植物研究。

收稿日期: 2010-09-30

网络出版日期: 2011-03-10

基金资助

福建省科技厅重点项目(2009N1003);福建省科技重大专项(2008NZ0002-1)

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Tissue Culture of Brassica alboglabra

CHEN Qiong-e ,
ZHONG Feng-lin ,
LIN Bi-ying

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1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002;
2. Vegetable Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002

Received date: 2010-09-30

Online published: 2011-03-10

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摘要

以登峰芥蓝为试材,取无菌苗的茎段、下胚轴为外植体分别进行组织培养研究。结果表明:MS+6-BA 3.0 mg/L+NAA 0.2 mg/L为下胚轴最适诱导培养基,MS+6-BA 5.0 mg/L+NAA 0.2 mg/L为带腋芽茎段最适诱导培养基;最适宜的不定芽增殖培养基为MS+6-BA 3.0 mg/L+NAA 0.2 mg/L;试管苗在1/2MS+NAA 0.1 mg/L+IBA 0.2 mg/L培养基中进行生根培养效果最好;经炼苗后组培苗移栽成活率达96.6%。

关键词： 芥蓝; 茎段; 下胚轴; 组织培养; 快速繁殖

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陈琼娥 , 钟凤林 , 林碧英 . 芥蓝组织培养研究[J]. 亚热带植物科学, 2011 , 40(01) : 34 -37 . DOI: 10.3969/j.issn.1009-7791.2011.01.009

Abstract

This experiment focused on testing the most suitable substrate for the tissue culture of Brassica alboglabra.The caulis segments and hypocotyl were separated from aseptic seedlings and used as explants.The results showed the best culture medium for hypocotyl was MS+6-BA 3.0 mg/L+NAA 0.2 mg/L, and for the caulis segments was MS+6-BA 5.0 mg/L+NAA 0.2 mg/L.The most suitable substrate for multiplication of adventitious buds was MS+6-BA 3.0 mg/L+NAA 0.2 mg/L, and the substrate with 1/2 MS+NAA 0.1 mg/L+IBA 0.2 mg/L had the best performance for rooting culture.The survival percentage of transplanting seedlings reached 96.6%.

Key words： Brassica alboglabra; stem segments; hypocotyl; tissue culture; rapid propagation

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