蝴蝶兰叶片蛋白质双向电泳方法初探

陈移亮

doi:10.3969/j.issn.1009-7791.2010.01.010

亚热带植物科学 >

2010 , Vol. 39 >Issue 01: 40 - 44

DOI: https://doi.org/10.3969/j.issn.1009-7791.2010.01.010

研究报告

蝴蝶兰叶片蛋白质双向电泳方法初探

陈移亮

展开

福建省亚热带植物研究所, 福建省亚热带植物生理生化公共实验室, 福建厦门 361006

陈移亮(1971-),男,福建德化人,助理研究员,从事植物蛋白质组学研究。

收稿日期: 2009-11-04

网络出版日期: 2010-03-10

基金资助

福建省自然科学基金(X0650022)

收起

Preliminary Studies on Two-dimensional Electrophoresis for Proteome of Phalaenopsis Leaves

CHEN Yi-liang

Expand

Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China

Received date: 2009-11-04

Online published: 2010-03-10

Fold

摘要

针对蝴蝶兰叶片蛋白质含量少且含有大量色素和酚等干扰物质的特点,通过对总蛋白提取方法、银染方法的改进,以及双向电泳实验条件的比较选择,初步建立一套适用于蝴蝶兰叶片蛋白质组分析的双向电泳技术。

关键词： 蛋白质组; 双向电泳; 蝴蝶兰

本文引用格式

陈移亮 . 蝴蝶兰叶片蛋白质双向电泳方法初探[J]. 亚热带植物科学, 2010 , 39(01) : 40 -44 . DOI: 10.3969/j.issn.1009-7791.2010.01.010

Abstract

Phalaenopsis leaves keep less protein and more non-protein substances like pigment,phenol and so on.To minimize the interference by non-protein substances,a 2-DE (two-dimensional electrophoresis) method which suited for the separation of protein from Phalaenopsis leaves was established in this experiment,on the basis of the total-protein extraction method and the silver dyeing method were modified and improved and the influential factors of 2-DE were compared and selected.

Key words： proteome; two-dimensional electrophoresis; Phalaenopsis

Options

文章导航

〈

〉

闽ICP备11008787号-5　

模态框（Modal）标题

摘要

本文引用格式

Abstract