兰花病毒病严重影响兰花产业的发展,研究和探索防治兰花病毒病的新技术、新途径已成为众多研究者普遍关注的焦点。本文综述目前抗兰花病毒研究中应用的各种抗病毒基因工程策略,包括病毒来源基因中的外壳蛋白基因和运动蛋白基因介导的抗性策略,RNA介导的抗病毒策略,植物自身的抗病毒基因介导的抗性策略,利用多基因介导的抗性策略,以及抗体基因介导的抗性策略等。最后对兰花抗病毒基因工程的发展及应用进行了展望。

The orchid virus disease seriously affects the development of orchid industry,research and exploration of orchid virus diseases prevention and control of new technologies,new ways to many researchers have become the focus of widespread concern.In this paper,the current anti-virus research in orchids of various anti-virus applications of genetic engineering strategies is summarized,which includes possible sources of the virus coat protein gene,the movement protein gene and gene-mediated resistance strategy,RNA-mediated anti-viral strategies,plants themselves anti-viral gene-mediated resistance strategy,multigene-mediated resistance strategy,and the use of antibody-mediated resistance strategy and so on.Finally,prospect in the development and application of orchid anti-virus genetic engineering is put forward.