取兰属大花蕙兰为母本、墨兰为父本进行种间杂交并进行杂种胚拯救研究。结果显示,子房离体培养明显优于胚珠离体培养,是胚抢救培养的有效手段。不同取材时间及在不同培养基中子房培养情况差异明显。在授粉后60 d子房离体培养即可获得成功,但以授粉后90 d子房离体培养最佳,萌发率高,达80%。子房培养的最适培养基为VW+BA 0.2 mg/L+NAA 0.2 mg/L+蛋白胨3 g/L+水解酪蛋白500 mg/L+椰子汁10%。

Interspecific hybridization was carried out with Cymbidium hybridium as female parent and C. sinense as male parent.Embryo rescue technique of hybrids was also studied.The results showed that ovaries culture was better than ovules culture, and ovaries culture had become to be effective means of embryo rescue.The germination and growth had significant difference at different growth stages and different culture medium.Ovaries could be cultured in vitro successfully as early as 60d after pollination.However, the germination rate was the highest when ovaries cultured for 90d after pollination, which reached up to 80%.Of the media studied, VW+BA 0.2 mg/L+NAA 0.2 mg/L+peptone 3 g/L+cascin hydrolyaate 500 mg/L+coconut milk 10% was the optimal medium for ovaries culture.