知母分蘖一步法快速繁殖体系的初步构建

洪森荣; 程细云; 周璐; 杨小露; 周芬

doi:10.3969/j.issn.1009-7791.2010.03.009

亚热带植物科学 >

2010 , Vol. 39 >Issue 03: 27 - 29

DOI: https://doi.org/10.3969/j.issn.1009-7791.2010.03.009

研究报告

知母分蘖一步法快速繁殖体系的初步构建

洪森荣 ,
程细云 ,
周璐 ,
杨小露 ,
周芬

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上饶师范学院生命科学学院, 江西上饶 334001

洪森荣(1974-),男,江西永新人,讲师,硕士,从事植物生物技术研究。

收稿日期: 2010-03-10

网络出版日期: 2010-09-10

基金资助

2009年江西省高校省级教改项目(JXJG-09-16-10)

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The Construction of One-Step Rapid Propagation System of Anemarrhena asphodeloides

HONG Sen-rong ,
CHENG Xi-yun ,
ZHOU Lu ,
YANG Xiao-lu ,
ZHOU Fen

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College of Life Sciences, Shangrao Normal University, Shangrao 334001, Jiangxi China

Received date: 2010-03-10

Online published: 2010-09-10

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摘要

以知母分蘖为试材,对知母的一步法快速繁殖体系进行了初步研究。结果表明,知母分蘖直接再生植株的最佳培养基是MS+KT 1 mg/L+NAA 0.5 mg/L;知母分蘖愈伤组织诱导和再生的最佳培养基是MS+KT 2 mg/L+NAA 0.5 mg/L。本实验构建了知母分蘖的一步法快速繁殖体系,为知母试管苗的工厂化生产提供了理论和技术基础。

关键词： 知母; 分蘖; 愈伤组织; 一步法快繁体系

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洪森荣 , 程细云 , 周璐 , 杨小露 , 周芬 . 知母分蘖一步法快速繁殖体系的初步构建[J]. 亚热带植物科学, 2010 , 39(03) : 27 -29 . DOI: 10.3969/j.issn.1009-7791.2010.03.009

Abstract

One-step rapid propagation system of Anemarrhena asphodeloides was studied with its tillers as experimental material.The results showed that the best direct regeneration medium of A. asphodeloides tiller was MS+KT 1 mg/L+NAA 0.5 mg/L, and the best callus induction and regeneration medium of A. asphodeloides tiller was MS+KT 2 mg/L+NAA 0.5 mg/L.The one-step rapid propagation system of A. asphodeloides tiller was built in this experiment, which would provide theoretical and technical basis for the factory production of A. asphodeloides plantlets.

Key words： Anemarrhena asphodeloides; tillers; callus; one-step rapid propagation system

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