海南疣粒野生稻DNA导入栽培稻及其后代的SSR验证

唐清杰; 云勇; 张吉贞; 严小微; 林尤珍; 韩义胜; 徐靖

doi:10.3969/j.issn.1009-7791.2010.04.003

亚热带植物科学 >

2010 , Vol. 39 >Issue 04: 7 - 9,13

DOI: https://doi.org/10.3969/j.issn.1009-7791.2010.04.003

研究报告

海南疣粒野生稻DNA导入栽培稻及其后代的SSR验证

唐清杰 ,
云勇 ,
张吉贞 ,
严小微 ,
林尤珍 ,
韩义胜 ,
徐靖

展开

1. 海南省农业科学院粮食作物研究所, 海南海口 571100;
2. 海南大学热带生物资源教育部重点实验室/海南大学海洋学院, 海南海口 570228

唐清杰(1981-),男,山东青岛人,助理研究员,硕士,从事稻种资源研究与利用。

收稿日期: 2010-07-08

网络出版日期: 2010-12-10

基金资助

国家科技支撑计划子课题(2009BADA2B001);海南省科学事业费项目(琼财预[2008]137号)

收起

Rice Transformation by Injecting Hainan Oryza meyeriana DNA and SSR Detection

TANG Qing-jie ,
YUN Yong ,
ZHANG Ji-zhen ,
YAN Xiao-wei ,
LIN You-zhen ,
HAN Yi-sheng ,
XU Jing

Expand

1. Cereal Crops Research Institute, Hainan Academy of Agricultural Sciences, Haikou 571100, Hainan China;
2. Key Laboratory of Tropical Biological Resources of Ministry of Education/The Ocean College of Hainan University, Haikou 570228, Hainan China

Received date: 2010-07-08

Online published: 2010-12-10

Fold

摘要

选取抗性较好且生长旺盛的海南疣粒野生稻,提取其基因组DNA,通过穗颈注射法将其导入杂交水稻恢复系R225、R128、R15。第1代开始选择在生育期、株高、株叶型和穗粒结构等性状与群体中其他单株有明显不同的变异株,经过3代选择,筛选到15份稳定材料。利用SSR分子标记检测4份导入后代材料具有供体而受体无的DNA条带,说明本试验获得了来自疣粒野生稻的变异材料。

关键词： 海南; 疣粒野生稻; 基因组DNA; 导入; SSR检测

本文引用格式

唐清杰 , 云勇 , 张吉贞 , 严小微 , 林尤珍 , 韩义胜 , 徐靖 . 海南疣粒野生稻DNA导入栽培稻及其后代的SSR验证[J]. 亚热带植物科学, 2010 , 39(04) : 7 -9,13 . DOI: 10.3969/j.issn.1009-7791.2010.04.003

Abstract

Genome DNA of Hainan Oryza meyeriana with high resistance and vigorous growth was extracted, then was transformed into rice restorer R225, R128, R15 by stem injecting.From F₁ generation, variation rice from others in growth period, height, type and spikelets was chosen.After 3 generation, 15 stable rice materials were screened out.The materials and controls were detected with SSR markers, which indicated that there were 4 materials from which DNA fragments specific to wild rice were amplified.The result showed that variation materials from Oryza meyeriana were acquired.

Key words： Hainan; Oryza meyeriana; genome DNA; transformation; SSR detection

Options

文章导航

〈

〉

闽ICP备11008787号-5　

模态框（Modal）标题

摘要

本文引用格式

Abstract