研究报告

多酚氧化酶抑制剂对蝴蝶兰叶外植体褐变的影响

  • 陈冬茵 ,
  • 赖艳艳 ,
  • 许传俊 ,
  • 李玲
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  • 1. 华南师范大学 生命科学学院, 广东省植物发育生物工程重点实验室, 广东 广州 510631;
    2. 福建省亚热带植物研究所, 福建 厦门 361006
陈冬茵(1987-),女,广东东莞人,本科生。

收稿日期: 2009-01-19

  网络出版日期: 2009-06-10

基金资助

国家大学生创新性实验计划项目(081057411);华南师范大学2008年度大学生创新性实验项目资助

Influences of PPO Inhibitors on Browning in Phalaenopsis Explants

  • CHEN Dong-yin ,
  • LAI Yan-yan ,
  • XU Chuan-jun ,
  • LI Ling
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  • 1. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, Guangdong China;
    2. Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China

Received date: 2009-01-19

  Online published: 2009-06-10

摘要

将多酚氧化酶(PPO)抑制剂添加到酶反应液中,抗坏血酸和半胱氨酸在0.5mmol/L就完全抑制蝴蝶兰PPO活性。300mg/L柠檬酸和100~200mg/L亚硫酸氢钠分别添加到培养基中,可使蝴蝶兰外植体褐变程度降低;采用抑制剂浸泡处理外植体,对外植体褐变抑制效果最好的为50mg/L抗坏血酸,外植体在褐变发生前PPO活性低于对照。

本文引用格式

陈冬茵 , 赖艳艳 , 许传俊 , 李玲 . 多酚氧化酶抑制剂对蝴蝶兰叶外植体褐变的影响[J]. 亚热带植物科学, 2009 , 38(02) : 15 -18 . DOI: 10.3969/j.issn.1009-7791.2009.02.004

Abstract

The effects of Polyphenol oxidase (PPO) activity inhibitors supplemented to reaction buffer at different concentrations showed that ascorbate acid and Cys complete inhibited PPO activity at 0.5 mmol/L. Adding PPO activity inhibitors to media at different concentrations, the results showed that 300 mg/L citric acid and 100-200mg/L NaHSO3 could reduce the browning of Phalaenopsis explants. Soaking explants with PPO activity inhibitors, it showed that 50 mg/L ascorbate acid gave the best result for browning inhibition and PPO activity was lower than that of control before explants browning happened.
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