研究报告

花榈木组培苗茎段低温胁迫培养及耐冷性诱导

  • 高丽 ,
  • 杨波 ,
  • 李洪林
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  • 中国科学院 武汉植物园, 湖北 武汉 430074
高丽(1979-),女,河南南阳人,硕士,从事植物生物技术研究。

收稿日期: 2008-12-03

  网络出版日期: 2009-06-10

基金资助

中国科学院知识创新工程重要方向性项目(KSCX2-YW-N-032)资助

Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro

  • GAO Li ,
  • YANG Bo ,
  • LI Hong-lin
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  • Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei China

Received date: 2008-12-03

  Online published: 2009-06-10

摘要

以花榈木组培苗茎段为试材,在低温胁迫条件下进行耐冷性愈伤组织诱导培养。结果表明,5℃是花榈木茎段低温胁迫培养及耐冷性诱导的最适温度。经过5℃低温胁迫培养获得的耐冷性愈伤组织在分化培养基MS+6-BA 2.0 mg/L+NAA 0.25 mg/L上的分化率达70.0%,且再生植株的低温伤害率明显低于对照。

本文引用格式

高丽 , 杨波 , 李洪林 . 花榈木组培苗茎段低温胁迫培养及耐冷性诱导[J]. 亚热带植物科学, 2009 , 38(02) : 19 -21,25 . DOI: 10.3969/j.issn.1009-7791.2009.02.005

Abstract

Stems of tissue culture seedlings of Ormosia henryi were cultured under low temperature stress. The results showed that the optimal temperature for induction of chilling tolerance callus was 5℃. The differentiation frequency of chilling tolerance callus cultured under 5℃ was 70.0% on MS solid medium supplemented with 6-BA 2.0 mg/L and NAA 0.25 mg/L, and the injury rate of regenerated plants from chilling tolerant callus was much lower than that of control group.
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