Abstract: Treated with 20% PEG (Polyethylene glycol)6000 (control)simulating drought stress and with 20% PEG 6000 following ABA pretreatment for 24h, respectively, characters of the spectrum band of POD isoenzyme in two turfgrasses (Common centipede grass and Bermudagrass)in 0~4d were studied. The results showed that the activity of POD in the leaves of Common centipede grass increased with the increasing time of PEG treatment and it decreased when treated with ABA. The effect of ABA pretreatment on the activity of POD in the leaves of Bermudagrass was not apparent. In control group, the band (at Rf 0.56)of POD isoenzyme of Common centipede grass disappeared with the increasing time of PEG treatment and a new band (at Rf 0.324)appeared after 4d of PEG treatment. With ABA pretreatment for 24h, the spectrum band (at Rf 0.327)was induced and some similar bands at Rf 0.22 were also induced.

Key words: ABA, PEG, POD isoenzyme, polyacrylamide gel electrophoresis

摘要： 以假俭草和狗牙根幼苗为材料,分别用PEG(聚乙二醇)处理和ABA预处理24h后再用PEG处理模拟干旱胁迫,研究在0~4d内POD同工酶电泳酶谱的结构及特征。结果表明,随着20%PEG处理时间延长,假俭草POD同工酶Rf 0.12和Rf 0.45区域的主要谱带颜色加深,POD活性提高。ABA预处理24h的假俭草POD同工酶谱带颜色却不同,其POD活性降低;而ABA预处理对狗牙根POD活性影响不明显。PEG处理后假俭草图谱中Rf值为0.56的酶谱随着胁迫时间延长而逐渐消失,但在处理第4d出现了新的Rf 0.324谱带,而ABA预处理24h就出现该谱带并一直存在;在Rf 0.22区域也存在几条类似的谱带。

关键词: ABA, PEG, POD同工酶, 聚丙烯酰胺电泳