Abstract: A method for determination of myclobutanil residues in Glycine max by gas chromatography was described.Samples were extracted with acetonitrile, followed by liquid-liquid partition and purified by neutral aluminum oxide column.The myclobutanil residues were analyzed by GC-ECD with a DB-1701 capillary column using N₂ as the carrier gas at a column temperature of 260℃ with an extensive standard, vaporizing chamber temperature of 240℃ and detector temperature of 300℃.The results showed that the method was quick and accurate with a good linear relative coefficient of 0.9998 in the range of 0.05~2.00 mg/L, average recoveries of 91.1%~99.0%, a variation coefficient of 1.22%~2.94%, a limit of detection of 1.0×10^-12 g, and a minimum determination concentration of 5.0×10^-4 mg/kg.

Key words: myclobutanil, residue, Glycine max, gas chromatograpy

摘要： 采用气相色谱法定量分析毛豆上腈菌唑的微量残留量。样品经乙腈提取,液液分配,中性氧化铝柱层析净化后,以气相色谱电子俘获检测器法(GC-ECD)测定,DB-1701毛细管柱、氮气为载气,柱温150℃20℃/min 260℃(10 min),气化室温度240℃,检测器温度300℃,外标法定量。该方法快速、准确,在0.05~2.00 mg/L范围内线性相关系数r²=0.9998,平均回收率91.1%~99.0%,变异系数1.22%~2.94%,最小检测量1.0×10^-12 g,最低检出浓度5.0×10^-4 mg/kg。

关键词: 腈菌唑, 残留, 毛豆, 气相色谱法