Abstract: Effects of 6-BA, NAA and KT on multiplication and differentiation of Anoectochilus roxburghii protocorm were studied by the way of L₁₆(4³) orthogonal design.The actions of 6-BA were extremely remarkable while NAA and KT were less effective.The best multiplication culture medium was improved KC+6-BA 2.0 mg/L+NAA 1.5 mg/L+KT 0.2 mg/L, the best differentiation culture medium was improved KC+6-BA 1.0 mg/L+NAA 0.5 mg/L.

Key words: Anoectochilus roxburghii, plant growth regulator, protocorm, multiplication, differentiation

摘要： 应用正交设计法探讨3种植物生长调节剂对花叶开唇兰原球茎增殖和分化的影响。结果表明,6-BA对原球茎增殖和分化作用显著,NAA和KT作用不显著;原球茎增殖最佳培养基为改良KC+6-BA 2.0 mg/L+NAA 1.5 mg/L+KT 0.2 mg/L,原球茎分化最佳培养基为改良KC+6-BA 1.0 mg/L+NAA 0.5 mg/L。

关键词: 花叶开唇兰, 植物生长调节剂, 原球茎, 增殖, 分化