Abstract: The experiment studied in the tissue culture of Phalaenopsis spp.. The results showed that in 1/2MS medium with 6-BA3.0mg/L+NAA0.2mg/L or with 6-BA 6.0mg/L, the induction rate of clustered shoots reached 100%. During the proliferating period of clustered shoots, the 1/2MS medium with 6-BA5.0mg/L+NAA0.5mg/L showed the best effects; in 1/2MS medium with 6-BA 15.0mg/L+NAA 1.0mg/L, the induction rate of protocorm-like body reached 20%, and in 1/2MS medium with 6-BA3.0mg/L+NAA0.5mg/L, the coefficient of reproduction is up to 7.4. It was easy for protocorm-like body differentiation to buds in 1/2MS+6-BA1.0mg/L+NAA0.5mg/L. In rooting stage, the 1/2MS medium with IBA0.3 mg/L was efficient. Transplanted them on water moor, and its survive rate reached 100% after two months. In addition, it was more conducive to the growth by water culture in the late cultivation.

Key words: Phalaenopsis spp., protocorm-like body, clustered shoots, tissue culture

摘要： 对蝴蝶兰丛生芽、原球茎途径的组织培养进行研究,结果表明,不经过愈伤组织直接诱导丛生芽的过程中,1/2MS+6-BA 6.0mg/L和1/2MS+6-BA 3.0mg/L+NAA 0.2mg/L两种培养基的诱导率可达100%,丛生芽增殖阶段采用1/2MS+6-BA 5.0mg/L+NAA 0.5mg/L效果最好;原球茎途径中,1/2MS+6-BA 15.0mg/L+NAA 1.0mg/L诱导率达20.0%,接入1/2MS+6-BA 3.0mg/L+NAA 0.5mg/L培养基中,增殖倍数达7.4,随后转入1/2MS+6-BA 1.0mg/L+NAA 0.5mg/L中,可很快分化成芽。生根培养基以1/2MS+IBA 0.3mg/L较好,将生根的蝴蝶兰移栽至水苔中,两个月后成活率达100%。此外,蝴蝶兰移栽6个月后转至水培更有利于生长。

关键词: 蝴蝶兰, 原球茎, 丛生芽, 组织培养