Subtropical Plant Science ›› 2011, Vol. 40 ›› Issue (01): 1-4.DOI: 10.3969/j.issn.1009-7791.2011.01.001

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Cloning Vernalization-Related Gene VRN2 and Construction of its Plant Expression Vector in Arabidopsis thaliana

ZHANG Wen-hui, HONG Li-ping, ZENG Bi-yu   

  1. Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China
  • Received:2010-09-29 Online:2011-03-10 Published:2011-03-10

拟南芥春化相关基因VRN2的克隆及其植物表达载体的构建

张文惠,洪丽萍,曾碧玉   

  1. 福建省亚热带植物研究所 福建省亚热带植物生理生化重点公共实验室, 福建 厦门 361006
  • 作者简介:张文惠(1972-),女,内蒙古呼盟人,副研究员,博士,从事基因工程、植物抗逆性及遗传育种研究。
  • 基金资助:

    福建省自然科学基金项目(2007J0250);福建省省属公益类科研院所基本科研专项(2009R10005-4)

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Abstract: In this paper, the vernalization-related Gene VRN2 cDNA from RNA of Arabidopsis thaliana L., GeneBank accession number AY063047 was successfully cloned.The cDNA sequence of VRN2 was 1 354 bp and its region in encoding was 1323 bp.The protein deduced from VRN2 contained 441 amino acids.The cloned cDNA of VRN2 was introduced into a middle vector pBPFΩ7.The fragment with P35S promoter and nos were digested by PstI, which were connected to pBI121 vector, giving plant espression vector containing VRN2 gene.

Key words: vernalization-related genes, VRN2, vernalization, Arabidopsis thaliana

摘要： 本文利用特异性引物,从拟南芥RNA中提取春化相关基因VRN2 cDNA序列,GenBank登录号AY063047。该基因序列大小为1 354 bp,编码区为1 323 bp,编码氨基酸441个。将克隆片段插入中间载体pBPFΩ7,经PstI酶切回收带有P35S启动子和nos终止子片段,连接pBI121载体,构建VRN2基因的植物表达载体。

关键词: 春化相关基因, VRN2, 春化作用, 拟南芥

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