Abstract: Shoot tips of ‘Highland No.7’ potato are selected as explants and 0.1% Mercuric Chloride and 75% alcohol are employed to disinfect; then through tissue culture, virus-free potato stems are obtained. In the process of enrichment culture, the paper tries to analyze the effect that 6-BA and KT with different consistency have on proliferation-inducing result of potato stems. The result indicates that it is the most effective to use 0.1% Mercuric Chloride to disinfect the shoot tips for 8 minutes, by which the disinfection rate can be up to 96.67%. The better promoting effect of potato stem proliferation made by cytokinin can be achieved when 6-BA > KT; adventitious bud induction and growth potential are the best when the consistency of 6-BA reaches 1.5 mg/L.

Key words: potato, shoot tip culture, stem, cytokinin

摘要： 以‘高原7 号’马铃薯茎尖为外植体，用0.1%升汞和75%酒精消毒，经组织培养获得脱毒马铃薯茎段，在增殖培养中，研究不同浓度6-BA 及KT 对马铃薯茎段诱导增殖效果的影响。结果表明：用0.1%升汞对茎尖消毒8 min 效果最好，灭菌率达96.67%。细胞分裂素对马铃薯茎段增殖的促进作用为6-BA>KT，以1.5 mg/L 6-BA 诱导不定芽及其生长势效果最佳。

关键词: 马铃薯, 茎尖培养, 茎段, 细胞分裂素