Abstract: The caryopsis of Dendrocalamus yunnanicus, which had been stored for 150 days at -20℃ and RH 15%, were used as the experimental materials, and aseptic clones were induced from them then used for propagation and in vitro conservation. The results showed that the best media for tufted-bud proliferation were MS + 6-BA 2 mg/L + NAA 0.2 mg/L, both sucrose and glucose contributed to the proliferation culture, and the best concentration was 3%. The most effective medium for rooting was MS + IBA 1 mg/L + NAA 1 mg/L, a single bud rooting was better; the best medium for buds in vitro conservation was MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L. The subculture cycle of shoots was 60 days at 25±3 ℃, which could change to 160 days and 240 days at low temperature preservation 20±3 ℃ and 12 ℃, respectively. Albino seedlings or variant of leaf colors were found during tissue culture.

Key words: Dendrocalamus yunnanicus, tufted-buds, rapid propagation, in vitro conservation

摘要： 本研究以干冷保存（-20 ℃，相对湿度15%）150 d 的云南龙竹（Dendrocalamus yunnanicus）颖果为实验材料，通过种子萌发建成丛芽无菌无性系进行快繁和离体保存。研究表明，丛芽增殖的最佳培养基为MS + 6-BA 2 mg/L + NAA 0.2 mg/L，蔗糖浓度以3%为佳；生根培养的最佳培养基为MS + IBA 1 mg/L + NAA 1 mg/L，以单芽诱导生根为好；离体保存丛芽的最适培养基为MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L；当培养温度由室温 (25±3)℃降低至(20±3)℃和12 ℃时，其继代周期可由原来的2 个月分别延长至4 个月和8 个月。在组织培养过程中发现白化苗或叶色变异现象。

关键词: 云南龙竹, 丛芽, 快速繁殖, 离体保存