Identification and Bioinformatics Analysis of the GRAS Transcription
Factor Family in Lycium ruthenicum

doi:10.3969/j.issn.1009-7791.2026.03.003

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Subtropical Plant Science ›› 2026, Vol. 55 ›› Issue (3): 282-295.DOI: 10.3969/j.issn.1009-7791.2026.03.003

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Identification and Bioinformatics Analysis of the GRAS Transcription Factor Family in Lycium ruthenicum

GUO Yong-chun¹, YANG Shi-min², LI Xiao-xiong³, MA Rui¹, MA Yan-jun^1*

(1. College of Forestry, Gansu Agricultural University, Lanzhou 730070, Gansu China; 2. Gansu Linxia Forest Pest Control and Quarantine Station, Linxia 731100, Gansu China; 3. Lanzhou Resources & Environment Voc-Tech University, Lanzhou 730030, Gansu China)

Received:2026-03-08 Accepted:2026-05-12 Online:2026-06-30 Published:2026-06-30
Contact: MA Yan-jun

黑果枸杞GRAS转录因子家族鉴定与生物信息学分析

郭咏淳¹，杨世民²，李效雄³，马瑞¹，马彦军^1*

(1. 甘肃农业大学林学院，甘肃兰州 730070；2. 甘肃省临夏州森林病虫害防治检疫站，甘肃临夏 731100；3. 兰州资源环境职业技术大学，甘肃兰州 730030)

通讯作者: 马彦军
基金资助:
甘肃省自然科学基金项目(24JRRG016)；甘肃省高校产业支撑计划项目(2023CYZC-46)；甘肃省白银市景泰县盐碱地综合利用试点项目(JTYJDSDXM-SG-02)；兰州市科技计划项目(2024-3-21)

Abstract

Abstract: In this study, the GRAS transcription factor family in Lycium ruthenicum was systematically analyzed using bioinformatics approaches. Through genome-wide screening and identification, a total of 28 GRAS family members were identified in L. ruthenicum. Phylogenetic analysis classified these members into five major subfamilies, namely DELLA, HAM, SHR, LISCL, and PAT1, revealing the evolutionary conservation and diversity of this family in L. ruthenicum. Subcellular localization prediction indicated that most of the GRAS proteins were localized in the nucleus. The GRAS proteins exhibited molecular weights ranging from 10.3 to 92.4 kDa, isoelectric points from 4.38 to 9.33, lengths from 39 to 823 amino acids, aliphatic indices from 58.11 to 129.40, and instability indices from 38.62 to 79.53. Among them, 24 members were predicted to be hydrophilic proteins, while the remaining were hydrophobic. All GRAS proteins contained the conserved VHIID domain at the C-terminus, with random coils and α-helices constituting the predominant secondary structural elements. Phylogenetic tree analysis revealed that L. ruthenicum is most closely related to tomato (Solanum lycopersicum). Expression profiling demonstrated that the transcript levels of LrGRAS7 and LrGRAS25 increased progressively with rising salt stress concentrations, suggesting their critical roles in the salt tolerance of L. ruthenicum. Collectively, these findings provide a theoretical basis for future functional studies of GRAS genes in L. ruthenicum.

Key words: Lycium ruthenicum, GRAS transcription factors, salt stress, gene expression

摘要： 利用生物信息学方法，对黑果枸杞GRAS转录因子家族进行系统性分析。通过全基因组筛选与鉴定，在黑果枸杞中共鉴定出28个GRAS家族成员。系统进化分析将这些成员划分为五个主要亚家族，包括DELLA、HAM、SHR、LISCL和PAT1，揭示了该家族在黑果枸杞中的进化保守性与多样性。亚细胞定位预测结果表明，黑果枸杞GRAS蛋白多数位于细胞核。黑果枸杞GRAS家族蛋白分子量10.3～92.4 kDa，等电点4.38～9.33，氨基酸个数39～823 aa，脂溶指数58.11～129.40，不稳定系数38.62～79.53；24个蛋白成员表现为亲水蛋白，其余为疏水蛋白。GRAS家族蛋白的C端具有VHIID保守结构域，无规则卷曲和α-螺旋占比较大。通过构建进化树可以得知，黑果枸杞与番茄亲缘关系最近。表达量分析表明，LrGRAS7、LrGRAS25基因表达量随盐胁迫浓度升高而逐渐提升，说明两者在黑果枸杞抗盐胁迫中发挥着关键作用。该研究结果为后续黑果枸杞GRAS基因功能研究提供理论依据。

关键词: 黑果枸杞, GRAS转录因子, 盐胁迫, 表达分析

CLC Number:

S567.1

GUO Yong-chun, YANG Shi-min, LI Xiao-xiong, MA Rui, MA Yan-jun. Identification and Bioinformatics Analysis of the GRAS Transcription Factor Family in Lycium ruthenicum[J]. Subtropical Plant Science, 2026, 55(3): 282-295.

郭咏淳, 杨世民, 李效雄, 马瑞, 马彦军. 黑果枸杞GRAS转录因子家族鉴定与生物信息学分析[J]. 亚热带植物科学, 2026, 55(3): 282-295.

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Identification and Bioinformatics Analysis of the GRAS Transcription Factor Family in Lycium ruthenicum

黑果枸杞GRAS转录因子家族鉴定与生物信息学分析

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