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10 March 2005, Volume 34 Issue 01 Previous Issue    Next Issue

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Effect of Methanol on Catalytic Activity of Papain CHU Zhi-zhan, HUANG Zhuo-lie, WU Guang-hong, HE Ping, ZHAN Fu-jian 2005, 34(01): 1-4,17.  Abstract ( )   PDF (531KB) ( )   The catalytic activity of papain could increase rapidly when treated with methanol. Results of kinetics experiments indicated that the value of Km and Vmax declined. The optimum pH of methanol solution was 8.5 and the optimum temperature was 70℃ for catalytic activity of papain. The experimental results of differential ultraviolet spectra of papain showed that the conformation of papain changed in methanol solution. The fluorescence emission spectra showed that the fluorescence intensity of papain increased and the fluorescence emission peak changed to lower wavelength in methanol solution. Related Articles | Metrics

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Changes of Membrane Fatty Acid Composition and Protein in Tea Leaves at Low Temperature YANG Ya-jun, ZHENG Lei-ying, WANG Xin-chao 2005, 34(01): 5-9.  Abstract ( )   PDF (456KB) ( )   The effects of low temperature on membrane fatty acid composition in leaves of 2 genotype tea trees (Camellia sinensis),Longjing 43 and Daye Yunfeng,were studied. The results showed that IUFA and the ratio of linolenic acid (18:3) of Longjing 43 tended to changing as ‘low-high-low’ pattern under low temperature treatment, and the membrane fatty acid composition of Daye Yunfeng changed irregularly at low temperature. The changes of protein in Longjing 43 leaves were also studied in the winter. The results indicated that the content and composition of soluble protein in leaves maintained stable. Under low temperature stress, the content of membrane proteins increased significantly, and two new membrane protein compositions,46KD and 38KD,appeared. The two protein compositions disappeared when the temperature had gone up. Related Articles | Metrics

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Optimization and Preliminary Application of ISSR-PCR Amplification for Cycas segmentifida MA Yong, LI Nan, ZHONG Ye-cong, LU Zhao-fu, LIN Jian-zhao, JIANG Ming, LIN Ping-yi, LUO Bin 2005, 34(01): 10-13.  Abstract ( )   PDF (385KB) ( )   Cycas segmentifida genomic DNA, extracted from its leaves by CTAB method, was used as ISSR-PCR amplification template. By adjusting the influencing factors of ISSR, such as template DNA concentration, dNTP concentration, Taq polymerase content, primer concentration, Mg2+ concentration and annealing temperature, the PCR amplication conditions were optimized. The optimal experiment conditions were as follows:PCR reaction volume of 20μl contained 2μl 10×PCR buffer, 20ng template DNA, 1U Taq polymerase, 1.5~2.75mmol/L MgCl2, 0.1mmol/L 4×dNTP, 0.22μmol/L primer and 2% formamide; proper annealing temperature was 50~52℃. 12 primers, which could produce more reproducible and polymorphic bands, were used to amplify all of the samples,and 122 DNA bands were generated, of which 86(70.49%) were polymorphic. POPGENE analysis indicated that moderate or slightly high levels of genetic diversity existed at species level, and the value of gene diversity coefficient(Gst) among populations was very high. Related Articles | Metrics

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Studies on Photosynthetic Characteristics of Gerbera hybrida var. diablo LI Yong-hua, WAN Guo-hui, YE Qing-sheng 2005, 34(01): 14-17.  Abstract ( )   PDF (309KB) ( )   The photosynthetic characteristics of leaves of Gerbera hybrida var. diablo under greenhouse conditions before, during and after flowering stages were studied. The results were as follows:Pn (about 8.23 CO2μmol/m2·s) under elevated CO2 concentration (600μmol/mol) was 27.1% higher than that under ambient CO2 concentration before flowering; the apparent quantum efficiency at flowering stage was 37.2% and 40.0% lower than that before flowering and after flowering, respectively, while the CO2 compensate point was 45.8% and 55.9% lower, respectively; the chlorophyll content in leaf after flowering was lower in comparison to those before and during flowering stages; the net photosynthetic rate at three stages had a similar value (about 6.0 CO2μmol/m2·s); the diurnal variation on net photosynthetic rate of Gerbera hybrida var. diablo in sunny days presented bimodal curve, the first peak occurred at about 11:00,which was 7.77 CO2μmol/m2·s, the second peak occurred at about 15:00 and the value was lower than the first one. Related Articles | Metrics

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Influence of Some Factors on DPPH· Scavenging Rate of Extraction of Gladiolus gandavensis Petals LONG Gang, ZENG You-wei, XU Liang-xiong, PENG Yong-hong 2005, 34(01): 18-20.  Abstract ( )   PDF (279KB) ( )   The influence of extracting temperature, solvent and time on activity of antioxidant substances in Gladiolus gandavensis petals were analyzed by using method of DPPH· free radical analysis. The results showed that the petal extraction had higher DPPH· scavenging rate when extracted with water or polar organic solvents for 60min at 70℃. Extraction of dark red petals and the petals growing at wilting stage had higher DPPH· scavenging rate. Related Articles | Metrics

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Comparative Photosynthetic Character between Vetiveria zizanioides and Its Companion Species Paspalum orbiculare LIU Jin-xiang, DENG Lian-fang, LIU Jia-qiong 2005, 34(01): 21-24.  Abstract ( )   PDF (344KB) ( )   The experiment of different photosynthetic active radiation (PAR) was conducted to study the photosynthetic physiological characters of Vetiveria zizanioides and Paspalum orbiculare, and the differences in photosynthesis rate (Pn), transpiration rate (Tr), water use efficiency (WUE) and stomatal conductance (Gs) were also evaluated. The results showed as follows:all the Pn, Tr and Gs increased with the enhanced PAR intensity, and the Pn, Tr and Gs of Paspalum orbiculare were significantly higher than those of Vetiveria zizanioides. But WUE of Vetiveria zizanioides was higher than WUE of Paspalum orbiculare,it indicating that Vetiveria zizanioides is dominant species and Paspalum orbiculare is merely auxiliary species. Related Articles | Metrics

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Relationship between Soil Nutrient of Newhall Orange Orchard and Fruit Quality BAO Jiang-feng, XIA Ren-xue, PENG Shu-ang, LI Guo-huai 2005, 34(01): 25-27.  Abstract ( )   PDF (270KB) ( )   The soil nutrient status (0~40cm) of Newhall orange orchards were determined in 2002 and their fruit qualities harvested in 2003 from Qualified Citrus Varieties Demonstration Farm in Zigui County (ZG-HB), Baopingcun Village of Gaoyang Town in Xingshan County(XS-HB), Leijiapingcun Village of Dongrangkou Town in Badong County (BD-HB) of Hubei Province were evaluated,respectively. The multiple linear stepwise regression analysis revealed that among a certain range, the TA content was negatively correlated with the content of soil organic matter, the ratio of TSS to TA was positively correlated with the content of available K, and the VitC was positively correlated with the content of available P. Related Articles | Metrics

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Effects of Exogenous GA3 and ABA Treatments on Fruit Quality of Cara Cara Orange WANG Gui-yuan, XIA Ren-xue 2005, 34(01): 28-30.  Abstract ( )   PDF (257KB) ( )   The effects of exogenous GA3 and ABA treatments on quality of Cara Cara orange before fruit coloring were studied. The results showed that GA3 treatment improved the rind brightness but decreased the rind redness, rind yellowness and individual fruit weight; ABA treatment improved the rind redness but decreased rind brightness and yellowness. Although 100mg/L GA3 treatment highly significantly decreased the rind thickness and increased rind brightness, total of soluble solid and vitamin C, it also decreased the rind redness, the ratio of sugar to acid and increased the acid content. Meanwhile,ABA treatment highly significantly reduced the percentage of edible part, percentage of fruit juice and VitC content. Exogenous GA3 and ABA treatments before fruit coloring were unfavourable to the quality of Cara Cara orange. Related Articles | Metrics

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A Preliminary Study on Mechanism of Oligosaccharin Inducing Rubber Tree Resistance to Powdery Mildew SHAN Jia-lin, XIAO Qian-chun, YU Zhuo-tong, HUANG Wu-ren, ZHENG Xue-qin, YANG Gong-zheng 2005, 34(01): 31-32,24.  Abstract ( )   PDF (442KB) ( )   The rubber leaf sprayed with oligosaccharin (OS) was compared to CK (water), including the leaf epidermal cell structure, the content of phenol and the activity of peroxidase. The results indicated that OS could induce the changes of tissue structure and the reaction of chemophysiology in rubber tree. It is the important mechanism of OS to improve the rubber tree resistance to powdery mildew. Related Articles | Metrics

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Karyotype Analysis of Chlorophytum comosum Chromosome LI Guo-tai 2005, 34(01): 33-35.  Abstract ( )   PDF (390KB) ( )   The karyotype and the number of Chlorophytum comosum chromosome were studied. The results showed that the number of chromosome of Chlorophytum comosum is 2n=2x=28=4m+14sm+10st; the relative length of chromosome is 2n=28=4L+14M2+10M1. The total length of chromosome groups is 73.84μm, the total length of long arms is 53.59μm, and the AS.K% is 72.58%. The karyotype of Chlorophytum comosum chromosome belongs to ‘3B’ type. The total volume of chromosome is 101.94μm3. Related Articles | Metrics

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Study on the Stability of Pineapple Juice CHEN Yi-nan 2005, 34(01): 36-38.  Abstract ( )   PDF (262KB) ( )   The product stability could be improved when certain stabilizing agents were added to the pineapple juice. The compound stabilizing agents were better for stability and taste of the drink than single stabilizing agent. Using an isotropic pressure of 2.5Mpa in the process of production and cooling the drink quickly after heating treatment gave a good result for the stability of pineapple juice. A moderate amount of acid and sugar had no obvious effects on the stability of the drink. Related Articles | Metrics

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Study on Seedling Growth and Raising Techniques of Prosopis abla GU Yun-jie, ZHOU Yong-li, GAN Shao-xiong, WU Zong-xing, LIU Fu-yun 2005, 34(01): 39-41.  Abstract ( )   PDF (297KB) ( )   Using 12 provenance seeds introduced from Argentina in 2002 as materials,the raising seedlings test of Prosopis abla was carried out at the Xuecheng Nursery in Wenchuan County, Sichuan Province. The differences of biomass, growth rhythm and phenophase among 12 provenance seeds at the seedling stage were evaluated. At the same time,test on seedling production with cutting root and apical bud was also conducted. The results showed that the cutting root could promote the growth of root system, and cutting apical bud was good for increasing the diameter of stem and the percentage of lateral branch. Related Articles | Metrics

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Induction Effect of Colchicine on the Root Tip Cell of Allium cepa HUANG Yong-lian, HU Mu-lin 2005, 34(01): 42-45.  Abstract ( )   PDF (316KB) ( )   The root tip cell of Allium cepa was treated with three kinds of colchicine at different concentrations. The results showed that colchicine could reduce the mitotic index of root tip cell of Allium cepa, promote the swelling of root tip, result in the doubling of chromosome and induce the cell aberration. Related Articles | Metrics

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Investigation and Study on Ornamental Plant Resources of Magnoliaceae in Fujian Province DENG Chuan-yuan, PAN Dong-ming, LAI Zhong-xiong 2005, 34(01): 46-49.  Abstract ( )   PDF (302KB) ( )   Ornamental plant resources of family Magnoliaceae in Fujian Province were investigated and studied. Family Magnoliaceae in Fujian Province comprises 9 genera and 35 species. The area types of species are mainly tropical distribution types. The species of genus Michelia are more than those of other genera. Leaves are mainly medium-sized and leathery. Flowers with fragrance are usually solitary and white,and appears in the summer. Fruits matures in late autumn and produces scarlet seeds. Almost all of the Fujian Magnoliaceae plants falls into trees and less shrubs, therefore, they have multiple landscaping uses. Related Articles | Metrics

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The Harmful Exotic Plants In Xiamen Area CHEN Heng-bin 2005, 34(01): 50-55.  Abstract ( )   PDF (416KB) ( )   63 species of harmful exotic plants belonging to 30 families, 53 genera in Xiamen area are described, and 12 important species are introduced in this paper. The origin area, life types and harmful degree of exotic plants are evaluated. The suggestions for controlling harmful exotic plants are also discussed. Related Articles | Metrics

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Plant Resources in Tanglangshan Suburb Park,Shenzhen City YANG Ji-ming, HE Zhong-jian, FENG Zhi-jian, TAN Guan-zhu 2005, 34(01): 56-59.  Abstract ( )   PDF (329KB) ( )   There exists 611 species belonging to 423 genera of 144 families of vascular plants in Tanglangshan Suburb Park, Shenzhen City. Among them, 33 species under 29 genera of 21 families are ferns;4 species under 3 genera of 3 families are gymnospermae and 574 species under 391 genera of 120 families are angiosperm. 7 species under 7 genera of 7families are national protected wild plants.5 species under 5 genera of 4 families are the rare and endangered plants, which accounts for 7% of 67 species in Guangdong Province.321 species are medicinal plants, 65 species are timer plants, 60 species are ornamental plants,41 species are fiber plants, 33 species are wild fruits, 38 species are oil plants, 42 species are forage plants, 42 species are tanning plants, 25 species are wild vegetables, 27 species are pesticide plants, 26 species are scent plants, 23 species are starch plants, 19 species are health plants, 13 species are dye plants, 10 species are venomousness plants, 5 species are honey-resource plants. Related Articles | Metrics

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Tissue Culture of Houttuynia cordata GONG Wei, HU Ting-xing, GONG Yuan-bo, ZHANG Jian, WANG Jing-yan 2005, 34(01): 60-62.  Abstract ( )   PDF (261KB) ( )   Taking stem segments with lateral buds as explants, the tissue culture of Houttuynia cordata were studied. The results showed that MS+6-BA 1.0mg/L+NAA 0.05mg/L was best for bud induction and the induction rate could reach 66.7% when cultured for 30d; MS+6-BA 2.0mg/L+2,4-D 0.2mg/L+NAA 0.2mg/L gave the best result for subculture of shoots and the multiplication rate could reach 7.4 times in 40d; MS+6-BA 3.0mg/L+2,4-D 0.5mg/L+NAA 0.1mg/L was best for increasing the weight of shoots and the fresh weight of shoots gain 7.358g in 40d; sand and vermiculite(1:1) was suitable for ex vitro shoots rooting and the rooting rate could reach 84%. Related Articles | Metrics

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Acquisition of Friable Calli from Leaves and Establishment of the Cell Suspension Culture of Tobacco ZHENG Yuan, XIAO Ya-ping 2005, 34(01): 63-63.  Abstract ( )   PDF (168KB) ( )   Related Articles | Metrics

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Primary Study on Tissue Culture of High Stalk Japanese Broccoli LAI Zheng-feng, LIN Jia-geng, LI Hua-dong, ZHENG Jia-xie 2005, 34(01): 64-64.  Abstract ( )   PDF (375KB) ( )   Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Lilium pollyanna YAN Zheng, GUO De-zhang 2005, 34(01): 65-65.  Abstract ( )   PDF (171KB) ( )   Related Articles | Metrics

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Cuttage Experiment of Clerodendranthus spicatus Shoot ZHANG Ping, TIE Wan-zhu, LAN Shi-kuan, LUO Guan-xing 2005, 34(01): 66-66.  Abstract ( )   PDF (356KB) ( )   Related Articles | Metrics

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A Review of Advances in Signal Transduction in Pollen Tubes during the Self-incompatibility Response of Higher Plants ZHENG Mao-zhong, TIAN Hui-qiao 2005, 34(01): 67-72.  Abstract ( )   PDF (384KB) ( )   Self-incompatibility (SI) is a genetically control mechanism that regulates the rejection of itself pollen to germinate. Some studies on the actin cytoskeleton and Ca2+alterations in pollen tube of Papaver rhoeas during SI response are reviewed in this paper. The signal transduction mediating the inhibition of pollen tube growth is also described. Related Articles | Metrics

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A Review of Recent Advances in Biotechnology Research on Loquat CAI Wen-yan, WU Shui-jin, XIAO Hua-shan 2005, 34(01): 73-76.  Abstract ( )   PDF (383KB) ( )   The advances in biotechnological research on loquat, including tissue culture, germplasm isolated preservation, protoplast culture and molecular marking are reviewed. The problems and the perspective of this field are also discussed. Related Articles | Metrics

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A Review of in vitro Induction of Polyploid Plants with Colchicine TAN De-guan, ZHUANG Nan-sheng, HUANG Hua-sun 2005, 34(01): 77-80.  Abstract ( )   PDF (307KB) ( )   The application of polyploid induction with colchicine in vitro culture is reviewed. The methods of induction, the optimum treatment combinations of colchicines, and the selection of inducing materials are summarized. It is suggested that the cultural techniques of polyploid plants has a great perspective for extension and application. Related Articles | Metrics