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10 June 2005, Volume 34 Issue 02 Previous Issue    Next Issue

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Making Template DNA in the Analysis of Tobacco Germplasm Based on AFLP Fingerprint YANG You-cai, ZHOU Qing-ming, YIN Han-qi 2005, 34(02): 1-4.  Abstract ( )   PDF (1060KB) ( )   Two methods-CTAB method and SDS method were tested in isolation of tobacco genomic DNA. The results showed that the degree of purity of DNA extracted by using CTAB method was higher than that by using SDS method; the value of OD260/OD280 for the DNA samples obtained with CTAB method was over 1.8; the molecular weight of DNAs was more than 23kb, the DNAs were completely digestible with EcoRⅠ/MseⅠ, and the AFLP figure were obtained. Related Articles | Metrics

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Cytohistological Studies on Morphogenesis Adventitious Buds in Tissue Culture of Lilium longiflorum YAO Shao-chang, AI Su-yun, YANG Mei-chun 2005, 34(02): 5-7.  Abstract ( )   PDF (1423KB) ( )   Sterilized leaf segments of lilium longiflorum were cultured for callus inducement and the results showed that MS+6-BA 2.0mg/L+NAA 0.2mg/L was the best for adventitious buds. The leaf segments were dissected at regular intervals to observe the initial places of dedifferentiation and histological changes during induced period. The results shows that the dedifferentiation initiates from wounded cells; the dedifferentiated cells form meristematic packed cells which were developed to be callus; the callus dedifferentiates and the adventitious buds form. The buds mainly develop from second to seventh layer cells of meristematic packed cells. Related Articles | Metrics

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Effect of Manganese on Some Physiological Characteristics of Soybean YANG De-jun, YANG Wei-yun, LIU Peng, LU Xiao-hong, XU Gen-di 2005, 34(02): 8-10.  Abstract ( )   PDF (811KB) ( )   The effect of manganese (Mn) on root activity, contents of proline, protein and malonaldehyde in leaves were studied using method of water culture. The results showed that appropriate Mn increased root activities of soybean and decreased contents of proline, protein and malonaldehyde in leaves, but the result was reversed under high Mn. There was difference in the response of soybean to Mn between Zhechun No.2 soybean and Zhechun No.3 soybean, and Zhechun No.3 was more sensitive than Zhechun No.2. Related Articles | Metrics

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Comprehensive Identification and Vigor Comparison of Agrobacterium rhizogenes TAO Jun, LI Ling 2005, 34(02): 11-14,24.  Abstract ( )   PDF (969KB) ( )   The comparison and identification of Agrobacterium strains (A4,R1205, R1000, R1601, R1022 and 15834) were investigated by 3-ketolactose production, differential acid production and motility test. The result indicated that R1205, R1601, R1000and A4 belonged to biotype Ⅱ and the vigor of strains was R1000 > R1205 > A4 > R1601. According to PCR assay, four strains (A4, R1205, R1000, R1601) were Agrobacterium rhizogenes. According to the transformatic model cucumber, which mediated by A4, R1205, R1000 and R1601, the transformatic frequency of R1000 was the highest (79.6%), and followed by R1205, A4 and R1601. Based on the experiment results, the vigor of R1000 was the highest. Related Articles | Metrics

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Effects of Chemical Treatment and Bagging on Fruit Quality of Cara Cara WANG Gui-yuan, XIA Ren-xue 2005, 34(02): 15-17.  Abstract ( )   PDF (794KB) ( )   The experiment was performed to study the effect of NaCl, MnSO4, NaH2PO4, GA3 treatment and bagging fruits after the second physiological fruit abscission on quality of Cara Cara fruit. The results showed that GA3 treatment increased the index of fruit shape, bagging could improve the rind brightness and yellowness extremely remarkably, but bagging without bag removal before fruit coloring decreased rind redness and weight of single fruit extremely remarkably; NaH2PO4 treatment increased the total of soluble solid remarkably, NaH2PO4 and GA3 treatment improved TA and decreased the ratio of TSS/TA. Bagging and bag removal before fruit coloring and MnSO4 treatment increased the total of soluble sugar extremely remarkably, bagging and bag removal before fruit coloring and NaH2PO4 treatment increased VitC content remarkably. Related Articles | Metrics

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Effects of Water Stress on Pollen Viability and Storability of Macadamia WANG Li-na, CHEN Li-lin, LIU Jian-fu, TANG Qing-lin 2005, 34(02): 18-20.  Abstract ( )   PDF (819KB) ( )   Under water stress treatments, the viability and storability of pollens collected from 3 varieties (Kau, Pahala and O.C.) of Macadamia were studied. The results showed that the viability of fresh pollen decreased with the storage time, and at room temperature the pollen could be stored for 6 days. Pollen viability decreased quickly after storing 6 days under water stress, with the increase of water stress, the pollen viability decreased more quickly. Under the different water stress treatments (i.e. saturated irrigation treatment, normal irrigation treatment, deficient irrigation treatment and CK), the storage life of pollen was 6d, 30d, 10d and 4d, respectively. The storability of 3 varieties was Kau >Pahala >O.C. and the best way to identifying pollen viability was biphenyl amine phenol. Related Articles | Metrics

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Effects of Mineral Nutrition on Some Bio-physiology Indexes of Narcissus tazetta var. Chinenese QIU Jin, YANG Sheng-chang, ZHONG Ran 2005, 34(02): 21-24.  Abstract ( )   PDF (887KB) ( )   Cultured Narcissus tazetta var. Chinenese in the solution added with mineral element, it was found that mineral nutrition could significantly improve the net photosynthetic rate and the ratio of photosynthesis to respiration, increase the content of soluble protein and chlorophyll in leaves in the stage of sprout and flowering period, as well as reduce the activities of SOD, POD and CAT, the production rate of O2· and MDA content of leaves in the stage of wilting. Mineral nutrition could effective prevent the over-oxidazition of membrane and the senescence of leaves. Related Articles | Metrics

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Application of the Principle Component Analysis on Mango Storage Characteristic ZHANG Zhen-wen, YAO Qing-qun 2005, 34(02): 25-28,33.  Abstract ( )   PDF (906KB) ( )   The principle component analysis(PCA) was used for statistic analysis on eight direction signs of post-harvest mangoes. The results showed that all the direction signs were vested in tow parts:one was storage characteristic factor, the other was physiological factor. The storage characteristic was composed of VitC content, titratable acid content, losing rate and chlorophyll content, and the physiological factor was composed of SOD activity, LOX activity and MDA content. Their dedicative rate was 63.69% and 32.46%, respectively, and the accumulative proportion for the tow factors was 96.16%. It reflected the information for all direction signs essentially. The integrated evaluate model on these basics also showed that the storage quality was improved a bit within 10d and then declined rapidly. These reflections were coincident with the facts basically. Related Articles | Metrics

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Effects of Municipal Domestic Sewage Effluents on the Growth of Calendula officinalis LIU Jun-wei, HU Hong-you, XU Gan-zhi, CHEN Deng-xiong, LU Chang-yi 2005, 34(02): 29-33.  Abstract ( )   PDF (1321KB) ( )   The seeds and potted plants of Calendula officinalis were treated with municipal domestic sewage effluents. The treatments were divided into 4 groups according to effluents:tap water(v/v):I, 1:0; Ⅱ, 1:0.5; Ⅲ, 1:1; control, 0:1. Compared with the control, sewage irrigation treatments (I, Ⅱ and Ⅲ) obviously reduced the rate of seed germination and increased the disease incidence of seedlings. The undiluted raw sewage could remarkably restrain the seedling growth, and the retarding effect could be relaxed by diluting with tap water. Furthermore, the diluted sewage of proper concentration could improve the seedlings growth. Compared to the control, sewage irrigation treatments increased the fresh weight of the stem, leave and root of full-grown C. officinalis plant by 161.63%~215.12%, 86.77%~109.23% and 23.89%~34.13%, respectively. The results of this study showed that the diluted sewage could be reused in irrigation and the nutrient elements in the sewage effluents could increase the ornamental value of herbage flowers. Related Articles | Metrics

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Effects of Weeding Compound Fertilizer “Dao-shui-bao” on Rice Production XIAN Hua-sheng, XIAN Hua-zhang, FAN Quan, ZHOU Yang 2005, 34(02): 34-35,38.  Abstract ( )   PDF (841KB) ( )   Field experiments of fertilizer application treatments (Ⅰ, weeding compound fertilizer ‘Dao-shui-bao’;Ⅱ, mixed fertilizer of 50% Butachlort+formulated fertilizer; Ⅲ, mixed fertilizer of 10% Bensulforon methyl+formulated fertilizer; Ⅳ, mixed fertilizer of 50% Butachlor+10% Bensulforonmethyl+formulated fertilizer; Ⅴ, CK) on rice were conducted. All the fertilizer application had the same concentrations of N, P2O5 and K2O. The results showed that treatment Ⅰ significantly increased the weeding efficiency and rice yield compared to the treatment Ⅲ and treatment Ⅳ, there were no significant differences between the treatment Ⅰ and treatment Ⅱ in rice production. The results also suggested that application of weeding compound fertilizer ‘Dao-shui-bao’ will result in savings of energy, reducing the costs and increasing profits, and is safe in rice production. Related Articles | Metrics

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Karyotype Analysis of Two Buckwheat Variety Chromosomes DU Xing, CHEN Min-yan, LIU Peng, XU Gen-di 2005, 34(02): 36-38.  Abstract ( )   PDF (917KB) ( )   The karyotype of two varieties of buckwheat GaoQiao 3 and XiQiao 5 were studied. The results showed that both the two varieties of buckwheat belong to ‘2A’ type. The karyotype of the buckwheat GaoQiao 3 is 2n=2x=16=10m(2SAT)+4sm+2st and the karyotype of the buckwheat XiQiao 5 is 2n=2x=16=8m+6sm(2SAT)+2st. Related Articles | Metrics

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Karyotype Analysis of Canna indica LI Guo-tai 2005, 34(02): 39-40.  Abstract ( )   PDF (841KB) ( )   The karyotye and the number of Canna indica chromosome were studied. The result showed that the number of chromosome of Canna indica is 2n=2x=18m; the karyotype of it belongs to ‘1A’ type. Related Articles | Metrics

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Measurement of Ursolic Acid Content in Clerodendranthus spicatus ZHANG Ping, HU Tan-lian, LUO Guan-xing, TIE Wan-zhu, LAN Shi-kuan 2005, 34(02): 41-42,45.  Abstract ( )   PDF (856KB) ( )   A TLCS method for determining the content of ursolic acid in Clerodendranthus spicatus was established in this experiment. The method was as follows. After extraction, the samples were dripped on silica gel GF254 TLC plate, using a mixture of the alkane-chloroform-acetic acid B ester-methyl alcohol (25:15:5:2) as developer and locating the position under the light of an UV lamp(254nm); double wavelength reflection sawtoothed scanning was performed λS=520nm, λR=650nm. The results showed that the regression equation is y=3658.34 X-7857.07, r=0.9998 and linear range is 0.5~2.5μg. The average recovery rate is 101.57%, RSD=2.83%. Related Articles | Metrics

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Extraction of Dicranopteris dichotoma Polysaccharides and Its Antibiotic Activities SU Yu-cai 2005, 34(02): 43-45.  Abstract ( )   PDF (839KB) ( )   Dicranopteris dichotoma polysaccharide was extracted by four methods,among those that the extracting rate of synthesis method was the highest,reaching 5.2%. The crude polysaccharide was purified by DEAE-52 and the neutral polysaccharide was eluted by distilled water and the polyanion polysaccharide was gradually eluted by 0.8mol/L NaCl. Their contents were 78% and 22%, respectively. The antibiotic activities of Dicranopteris dichotoma were tested with the agar disc diffusion method. The result showed that the polysaccharide had various amount of antibiotic activities against bacteria and fungi. Related Articles | Metrics

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The Study on the Extraction Method of Indirubin from Root of Isatis indigotica CHENG Li-hui 2005, 34(02): 46-47.  Abstract ( )   PDF (768KB) ( )   Comparative experiment of decocting method with infiltration in extracting effective component from banlangen was conducted by TLC. The old method was improved to increase the extraction rate of indirubin. Related Articles | Metrics

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An Elementary Report on Introduction of Six Early-Maturing and Tasty Eurasia Grape Varieties LIU Yu-rong, LIN Song-cang, YANG Hui-chang, ZHENG Yi-qing, WEI Ze-ping 2005, 34(02): 48-50.  Abstract ( )   PDF (796KB) ( )   Taking a sample of six early-maturing, tasty Eurasia grape varieties for experiment, their phenological phase, fruit economic properties, disease resistance, secondary fruit growth and yield under the equipped circumstances of rain prevention were studied. The results showed that among these six varieties there were obvious differences in their yield, quality and disease resistance. By comparison of comprehensive properties, Jing-Xiu is the best and the following one is Ogoust, and these two varieties are valuable for popularization in Lingde City. Related Articles | Metrics

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Effects of Cold Storage and Culture Style on the Flowering Regulation of Iris×hollandica LIN Bing, HUANG Min-ling, YE Yi-yuan, WU Jian-she, YE Xiu-xian 2005, 34(02): 51-52.  Abstract ( )   PDF (775KB) ( )   Related Articles | Metrics

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The Identification and Geographic Distribution of Nageia and Its Pharmaceutical Effect SUN Tong-xing, WANG Xue-ying 2005, 34(02): 53-55.  Abstract ( )   PDF (828KB) ( )   The morphological character and geographical distribution of Nageia are described. The chemical composition of Nageia and its medicinal value are introduced. Related Articles | Metrics

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Investigation and Exploitation on Resources of Wild Poisonous Plants in Kunyu Mountain,Shandong Province ZHAO Hong, HAN Xiao-di, ZHANG Wei 2005, 34(02): 56-59,47.  Abstract ( )   PDF (865KB) ( )   The poisonous wild plants in Kunyu Mountain, Shandong Province, about 94 species, 48 families are described. They are about 15% of medical plants in Kunyu Mountain. The resources of these plants are evaluated. It provides an important data for development and utilization of the resources. Related Articles | Metrics

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Distribution and Medical Value of Rodgeris aesculifolia and Its Exploitation ZHANG Xiao-yan, LI Duo-wei, WANG Yi-chao, SUN Shi-qing 2005, 34(02): 60-62.  Abstract ( )   PDF (815KB) ( )   Species, distribution, medical value and utilization of Rodgeris aesculifolia are summarized. Some suggestions for its development and utilization are also presented. Related Articles | Metrics

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Study on 60Coγ Radiate Mutation in Breeding of phalaenopsis ZHANG Ning, SU Ming-hua, LIU Fu-ping, LIN Qing-hong 2005, 34(02): 63-63.  Abstract ( )   PDF (784KB) ( )   Related Articles | Metrics

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High-efficient Regeneration System of Torenia fournieri from Root Section Induced by TDZ KANG Pei-jing, LI Mei-lan, LI Hong-qing 2005, 34(02): 64-64.  Abstract ( )   PDF (738KB) ( )   Related Articles | Metrics

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Tissue Culture of Stephanotis floribunda ZHANG Shu-he, ZHOU Long-sheng, LIN Jia-geng, Wu Wei-jian 2005, 34(02): 65-65.  Abstract ( )   PDF (733KB) ( )   Related Articles | Metrics

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A Review of the Advances in Cytokinin Biosynthesis ipt Gene WU Ji-lin, WANG Zai-hua, YE Qing-sheng, LI Ling 2005, 34(02): 66-69,80.  Abstract ( )   PDF (904KB) ( )   Isopentenyl-transferases catalyze the first and rate-limiting steps of cytokinin biosynthesis, and the corresponding genes have been cloned. A family of genes from Arabidopsis coding for cytokinin biosynthesis enzymes have been identified by a bioinformatic approach. It is speculated that these genes might be expressed in distinct spatial and temporal patterns to regulate cytokinin biosynthesis. This review specially introduced the functions and advances of ipt in cytokinin biosynthesis. Related Articles | Metrics

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Progress in Chemical Constituents of Nyssaceae Family HUANG Jiang, LIU Rong, WANG Cong-zhou, OUYANG Ming-an 2005, 34(02): 70-75.  Abstract ( )   PDF (992KB) ( )   Tens of compounds were isolated from the plants belonging to Nyssaceae family, most of them with novel skeletons showing biological activities. The characteristic constituents were classified as quinline alkaloids, indole alkaloids, ellagic acid derivatives, flavones and others. This article summarized the structures and spectral data of the compounds and their pharmacological activities. Related Articles | Metrics

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A Review of Study on Sod Cultivation in Fruit Orchard LIU Chuan-he, CHEN Jie-zhong 2005, 34(02): 76-80.  Abstract ( )   PDF (860KB) ( )   The sod cultural practices and patterns in fruit orchard are described in this paper. The effects of sod cultivation on the soil and water conservation, the improvement of orchard environment, the physico-chemical and microbiological characters of soils are discussed. And the effects of sod cultivation on the development and growth of fruit trees, the quality and yield of fruits are evaluated. Related Articles | Metrics