亚热带植物科学 ›› 1995, Vol. 24 ›› Issue (02): 10-15.
• 研究报告 • 上一篇 下一篇
刘新民,林耀辉,陈移亮,沈育芬
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Liu Xinmin, Lin Yaohui, Chen Yiliang, Shen Yufen
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摘要： 用海藻酸钠、二醋酸纤维、明胶分别作为固定化果胶酶的载体,研究比较载体的用量、交联剂的浓度、用酶量、制备的pH。结果表明,选择15%浓度的明胶为载体,5%浓度的戊二醛为交联剂,用酶量5%,在pH 3.5条件下制备固定化果胶酶,其酶活力回收率可达67.74%,重复回收使用10次后,酶活力还可保留80%以上,是果胶酶固定化的一种较好的方法。
关键词: 果胶酶, 固定化, 载体
Abstract: Sodium alginate,diacetate fibre and gelatin were used as carriers respec-tively for immobilization of pectinase.A comparison was made among the preparing con-ditions,i.e. the dosage of carrier,the concentration of cross linking agent,the dosage of pectinase and pH. The results showed that the recovery ratio of pectinase activity was up to 67.74% when the immobilized pectinase was prepared with 15% gelatin as carri-er,5% glutaric acid as cross linking agent and 5% pectinase at pH 3.5,and the enzyme activity retained more 80% after recovering 10 times repeatedly.This suggests that gela-tinization is a better method for immobilization of pectinase.
Key words: Pectinase, Immobilization, Carrier
刘新民,林耀辉,陈移亮,沈育芬. 果胶酶固定化方法的研究[J]. 亚热带植物科学, 1995, 24(02): 10-15.
Liu Xinmin, Lin Yaohui, Chen Yiliang, Shen Yufen. Methods for Immobilization of Pectinase[J]. Subtropical Plant Science, 1995, 24(02): 10-15.
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