毛豆根瘤菌转吸氢基因的研究

亚热带植物科学 ›› 1996, Vol. 25 ›› Issue (01): 13-16.

毛豆根瘤菌转吸氢基因的研究

龙敏南,张凤章,许良树

厦门大学生物系, 厦门 361005

收稿日期:1995-12-27 出版日期:1996-03-10 发布日期:1996-03-10
基金资助:
福建省自然科学基金

Translation of Hydrogen Uptake Genes into Rhizobium japonicum

Long Minnan, Zhang Fengzhang, Xu Liangshu

Department of Biology, Xiamen University, Xiamen 361005

Received:1995-12-27 Online:1996-03-10 Published:1996-03-10

摘要/Abstract

摘要： 通过三亲本杂交将携带豌豆根瘤菌吸氢基因的质粒pAL₆₁₈转移到台湾毛豆根瘤菌292-C中,经抗性筛选、质粒检测和吸氢活性测定,得到能稳定遗传的结合株292-C₂和292-C₃。在自生条件下结合株均表现较高的吸氢活性,而受体株292-C不吸氢,在共生条件下结合株表现为高吸氢,而受体株表现为放氢。

关键词: 台湾毛豆根瘤菌, 吸氢基因, 吸氢活性

Abstract: The cosmid pAL₆₁₈ containing the hydrogen uptake genes of Rhizobium leguminosarum was transferred into Rhizobium Japonicum (Glycine max meer 292-C) by triparent mating system,Two transconjugants 292-C₂ and 292-C₃ were selected in Rhizobium mininal medium containing Ampicillin (25 mg/L) and Tetraeycline (10 mg/L).The expression of hydrogenase activities of transconjugants were studied.The transconju-gants 292-C₂ and 292-C3 showed high hydrogenase activity both in free-living and symbi-otic state whiie the recipient showed no hydrogen uptake activity in free-living state and hydrogen evolution in symbiotic state.

Key words: Rhizobium japonicum, Hydrogen uptake genes, Hydrogenase activity

龙敏南,张凤章,许良树. 毛豆根瘤菌转吸氢基因的研究[J]. 亚热带植物科学, 1996, 25(01): 13-16.

Long Minnan, Zhang Fengzhang, Xu Liangshu. Translation of Hydrogen Uptake Genes into Rhizobium japonicum[J]. Subtropical Plant Science, 1996, 25(01): 13-16.