亚热带植物科学 ›› 1998, Vol. 27 ›› Issue (02): 17-20.

• 研究报告 • 上一篇    下一篇

高效液相色谱法分离测定香兰素

黄玉环,刘鸿洲,尤瑞琛   

  1. 福建省亚热带植物研究所省重点公共实验室, 厦门 361006
  • 收稿日期:1998-03-03 出版日期:1998-06-10 发布日期:1998-06-10

Determination of Vanillin in Vanilla Beau by HPLC

Huang Yuhuan, Liu Hongzhou, You Ruichen   

  1. Fujian Institute of Subtropical Botany, Xiamen 361006
  • Received:1998-03-03 Online:1998-06-10 Published:1998-06-10

摘要: 用60%乙醇提取香荚兰豆的香兰素,经高效液相色谱法分离,在紫外波长330 nm处测定。选取适当的流动相,能快速、准确地测定出香兰素,最低检测含量可达到0.01 μg。

关键词: 高效液相色谱(HPLC), 香兰素, 检测

Abstract: Vanillin was extracted from vanilla bean with 60% ethyl alcohol, and then separated by HPLC and determined at UV 330 nm. This method offers a new approach simplifying the treatment of samples and saving time as well as lowering operating costs. The lowest limit of vanillin content of vanilla bean using this method was 0. 01 μg.

Key words: HPLC, Vanillin, Determination