甘蔗花叶病毒P1基因的原核表达及其抗体制备

doi:10.3969/j.issn.1009-7791.2009.04.001

亚热带植物科学 ›› 2009, Vol. 38 ›› Issue (04): 1-4.DOI: 10.3969/j.issn.1009-7791.2009.04.001

• 研究报告 • 下一篇

甘蔗花叶病毒P1基因的原核表达及其抗体制备

郭莺^1,2,阮妙鸿²,刘佳²,杨川毓²,张木清²

1. 福建省亚热带植物研究所, 福建厦门 361006;
2. 农业部甘蔗生理生态与遗传改良重点开放实验室, 福建福州 350002

收稿日期:2009-08-03 出版日期:2009-11-10 发布日期:2009-11-10
作者简介:郭莺(1981-),女,福建龙岩人,博士,助理研究员,从事植物抗病分子育种研究。
基金资助:
国家自然科学基金项目(30871514)

Expression and Antibody Preparation of Sugarcane Mosaic Virus P1 Protein

GUO Ying^1,2, RUAN Miao-hong², LIU Jia², YANG Chuan-yu², ZHANG Mu-qing²

1. Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China;
2. Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, Fuzhou 350002, Fujian China

Received:2009-08-03 Online:2009-11-10 Published:2009-11-10

摘要/Abstract

摘要： 将扩增的甘蔗花叶病毒P1基因克隆到原核表达载体pET-32a上,转化BL21(DE3),获得重组子pET-32a-P1。超声波结果显示,所得的融合蛋白以可溶性的形式存在。SDS-PAGE检测其表达产物与目的蛋白大小一致,割胶免疫注射家兔得到抗体。间接ELISA测定结果表明稀释51200倍仍呈阳性信号,并通过western blot检测,证明抗体特异性良好。

关键词: 甘蔗花叶病毒, P1, 融合蛋白表达, 抗体

Abstract: The target gene was subcloned into the prokaryotic expression vector pET-32a,and transformed into E. coli Bl21(DE3),to obtain recombinant pET-32a-P1.Ultrasound results showed that the fusion protein existed in soluble form.Expression product had the same size with the target protein detected by SDS-PAGE.The antibody against the P1 protein was gained by immunized rabbit with target protein cut from SDS-PAGE.Indirect ELISA measurements proved that the antibody specificity was fine,because of that the dilution of 51200-fold not merely kept positive still,but also passed western blot.

Key words: Sugarcane mosaic virus, P1, fusion protein expression, antibody

中图分类号:

Q786

郭莺,阮妙鸿,刘佳,杨川毓,张木清. 甘蔗花叶病毒P1基因的原核表达及其抗体制备[J]. 亚热带植物科学, 2009, 38(04): 1-4.

GUO Ying, RUAN Miao-hong, LIU Jia, YANG Chuan-yu, ZHANG Mu-qing. Expression and Antibody Preparation of Sugarcane Mosaic Virus P1 Protein[J]. Subtropical Plant Science, 2009, 38(04): 1-4.

甘蔗花叶病毒P1基因的原核表达及其抗体制备

Expression and Antibody Preparation of Sugarcane Mosaic Virus P1 Protein

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