关键词: 石蒜属, ITS, 亲缘关系

Abstract: The inter-species relationship of 13 species in the genus Lycoris was studied based on sequences of the internal transcribed spacer (ITS) of nuclear ribosomal DNA.Total DNAs of Lycoris were extracted from silica-dried leaves of each species.The ITS sequences were amplified by PCR with universal primer of ITS and PCR products were sequenced after purification.The length of ITS1 and ITS2 sequences were 259~260 bp and 230 bp respectively.There were distinct variant sites between the ITS sequences.Phylogenetic analysis showed that Lycoris could be classified into three clades:clade I (M+T) with L.chinesis, L.aurea, L.anhuiensis and L.longituba, clade II (ST) with L.radiata var.punila, L.sprengeri, L.rosea and L.haywardii, and clade III (ST+T+M) with L.straminea, L.albifora, L.caldwellii and two Hydrids.The result was basically consistent with that of chromosomekayotype study.The clade III was presumed from interspecific hybridization between two other clades.

Key words: Lycoris, internal transcribed spacer (ITS), phylogeny