毛豆中腈菌唑残留量的气相色谱法测定

亚热带植物科学 ›› 2006, Vol. 35 ›› Issue (03): 48-50.

毛豆中腈菌唑残留量的气相色谱法测定

陈丽萍¹,林志平²

1. 福建省漳州市农业检验监测中心, 福建漳州 363000;
2. 漳州市植物检疫监测中心, 福建漳州 363000

收稿日期:2006-02-12 出版日期:2006-09-10 发布日期:2006-09-10
作者简介:陈丽萍(1972-),女,福建龙岩人,农艺师,从事农药残留分析。
基金资助:
漳州市科技计划项目(Z03028)

Determination of Myclobutanil Residues in Glycine max by Gas Chromatography

CHEN Li-ping¹, LIN Zhi-ping²

1. Zhangzhou Agricultural Test and Inspection Center, Zhangzhou 363000, Fujian China;
2. Zhangzhou Plant Quarantine and Inspection Center, Zhangzhou 363000, Fujian China

Received:2006-02-12 Online:2006-09-10 Published:2006-09-10

摘要/Abstract

摘要： 采用气相色谱法定量分析毛豆上腈菌唑的微量残留量。样品经乙腈提取,液液分配,中性氧化铝柱层析净化后,以气相色谱电子俘获检测器法(GC-ECD)测定,DB-1701毛细管柱、氮气为载气,柱温150℃20℃/min 260℃(10 min),气化室温度240℃,检测器温度300℃,外标法定量。该方法快速、准确,在0.05~2.00 mg/L范围内线性相关系数r²=0.9998,平均回收率91.1%~99.0%,变异系数1.22%~2.94%,最小检测量1.0×10^-12 g,最低检出浓度5.0×10^-4 mg/kg。

关键词: 腈菌唑, 残留, 毛豆, 气相色谱法

Abstract: A method for determination of myclobutanil residues in Glycine max by gas chromatography was described.Samples were extracted with acetonitrile, followed by liquid-liquid partition and purified by neutral aluminum oxide column.The myclobutanil residues were analyzed by GC-ECD with a DB-1701 capillary column using N₂ as the carrier gas at a column temperature of 260℃ with an extensive standard, vaporizing chamber temperature of 240℃ and detector temperature of 300℃.The results showed that the method was quick and accurate with a good linear relative coefficient of 0.9998 in the range of 0.05~2.00 mg/L, average recoveries of 91.1%~99.0%, a variation coefficient of 1.22%~2.94%, a limit of detection of 1.0×10^-12 g, and a minimum determination concentration of 5.0×10^-4 mg/kg.

Key words: myclobutanil, residue, Glycine max, gas chromatograpy

中图分类号:

X592

陈丽萍,林志平. 毛豆中腈菌唑残留量的气相色谱法测定[J]. 亚热带植物科学, 2006, 35(03): 48-50.

CHEN Li-ping, LIN Zhi-ping. Determination of Myclobutanil Residues in Glycine max by Gas Chromatography[J]. Subtropical Plant Science, 2006, 35(03): 48-50.

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