关键词: 白芨, 胚培养, 无菌播种, 快速繁殖

Abstract: With the increasing age of Bletilla Striata embryo, the maturity of embryo was enhanced, and the rate of albuminous seed and the sprouting rate of seed increased, while the sprouting time began to shorten. It was the best time to pick the capsules when the embryo had attained the age of 20wk, thus, giving the highest albuminous seed rate and 100% sprouting rate of seed, and it merely took one week to sprout. After germination, the embryos first developed into PLBs, then multiplicated and differentiated into leaves and roots, and formed complete plantlet. The medium added with Huabao No.1 1g/L+Huabao No.2 2g/L+10% coconut milk gave the best result for seeds sprouting and seedlings growth. The medium was more simple and convenient than the general medium supplemented with growth regulator.

Key words: Bletilla striata, embryo culture, germfree sowing, rapid propagation