亚热带植物科学 ›› 2001, Vol. 30 ›› Issue (01): 24-26.

• 研究报告 • 上一篇    下一篇

木薯离体培养与快速繁殖

杭玲,陈丽娟,蔡炳华,苏国秀,程汤,黄卓忠   

  1. 广西农业科学院 生物技术研究所, 广西 南宁 530007
  • 收稿日期:2000-09-11 出版日期:2001-03-10 发布日期:2001-03-10
  • 作者简介:杭玲(1953-),女,广西南宁人,副研究员,现从事植物细胞工程组织培养研究。

Culture in vitro and rapid propagation of Cassava

HANG Ling, CHEN Li-juan, CAI Bing-hua, SU Guo-xiu, CHENG Tang, HUANG Zhuo-zhong   

  1. Biological Technological Institute, Guangxi Academy of Agricultural Science, Nanning 530007, Guangxi China
  • Received:2000-09-11 Online:2001-03-10 Published:2001-03-10

摘要: 选取3个木薯品种的腋芽作为外植体,分别接种于MS基本培养基上进行离体培养及快速繁殖。结果表明, 6-BA不同浓度对木薯品种不定芽诱导效果不一,在MS+6-BA 0.5~1.0mg/L中,3个品种均可诱导产生幼芽;在MS+6-BA 3.0mg/L中,3个品种的幼芽诱导率高达100%;MS+6-BA 3.0mg/L+NAA 0.1mg/L对继代效果较好;MS+NAA 0.1mg/L对生根效果最佳。

关键词: 木薯, 离体培养, 快速繁殖

Abstract: Explants from axillary buds of three varieties of Cassava (Manihot esculenta) were cultured on MS media. The results suggested that various concentrations of 6-BA had different effects in inducing adventitious buds. The young shoots of three varieties could be produced on MS+6-BA 0.5~1.0mg/L and their inducing rate was up to 100% on MS+6-BA 3.0mg/L; MS medium supplemented with 6-BA 3.0mg/L and NAA 0.1mg/L gave good result in subculture for rosette buds and MS+NAA 0.1mg/L gave best result for rooting.

Key words: Manihot esculenta, culture in vitro, rapid propagation

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