亚热带植物科学 ›› 2001, Vol. 30 ›› Issue (01): 32-35.

• 研究报告 • 上一篇    下一篇

绿巨人叶柄离体培养及植株再生

张文珠,林德钦,李梅   

  1. 厦门华侨亚热带植物引种园, 福建 厦门 361002
  • 收稿日期:2000-10-20 出版日期:2001-03-10 发布日期:2001-03-10
  • 作者简介:张文珠(1972-),女,福建尤溪人,研究实习员,学士,从事花卉脱毒种苗的快速繁殖研究。

Tissue culture and plantlet regeneration from petiole of Spathiphyllum floribundum cv. Maura Loa

ZHANG Wen-zhu, LIN De-qin, LI Mei   

  1. Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, Fujian China
  • Received:2000-10-20 Online:2001-03-10 Published:2001-03-10

摘要: 在MS基本培养基中添加不同植物生长调节剂(mg/L)对绿巨人无菌苗的叶柄进行离体培养,结果表明,MS+6-BA 1.0+NAA 2.0+2,4-D 1.0诱导效果最好,产生愈伤组织并分化出丛芽;MS+6-BA 2.0~3.0+NAA 0.2+0.2% PVP对芽的增殖效果好;附加0.2%PVP对防止褐变有一定效果;生根培养基选用1/2MS+NAA 0.1~0.5+IBA 1.0的组合。

关键词: 绿巨人, 叶柄, 离体培养, 植株再生

Abstract: Petioles of Spathiphyllum floribundum cv. Maura Loa were cultured on MS medium supplemented with different concentration hormones. The results showed that MS medium containing 6-BA at 1.0mg/L, NAA at 2.0mg/L and 2,4-D at 1.0mg/L gave best results in callus induction; a combination of MS with 6-BA at 2.0~3.0mg/L, NAA at 0.2mg/L and 0.2% PVP was good for proliferation of adventitious buds; MS supplemented with 0.2% PVP could prevent from browning; and 1/2MS medium containing NAA at 0.1~0.5mg/L and IBA at 1.0mg/L was favorable for rooting.

Key words: Spathiphyllum floribundum cv. Maura Loa, petiole, tissue culture, plantlet regeneration

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