›› 2017, Vol. 46 ›› Issue (02): 122-125.DOI: 10.3969/j.issn.1009-7791.2017.02.005

• 植物生理生化与分子生物学 • 上一篇    下一篇

降低卷丹百合组培褐变技术研究

梁春辉,闫晓东,黄敏,李荣喜,陈礼芬,李秀平   

  1. (1.广东农工商职业技术学院热作系,广东 广州 510507;2.广州出入境检验检疫局,广东 广州 510623)
  • 收稿日期:2017-04-14 修回日期:2017-05-09 出版日期:2017-06-30 发布日期:2017-06-30
  • 通讯作者: 李秀平
  • 作者简介:梁春辉,实验师,从事植物组织培养研究。
  • 基金资助:
    广东省高等职业教育作物生产技术品牌专业建设项目[粤教高函(2015)191号];广东省高等职业教育园林技术品牌专业建设项目[粤教高函(2016)293号]

Techniques of Reducing Tissue Culture Browning of Lilium lancifolium

LIANG Chun-hui,YAN Xiao-dong,HUANG Min,LI Rong-xi,CHEN Li-fen,LI Xiu-ping   

  1. (1.Tropical Crops Department, Guangdong Agriculture Industry Business Polytechnic College, Guangzhou 510507, Guangdong China; 2.Guangzhou Entry-exit Inspectional and Quarantine Bureau, Guangzhou 510623, Guangdong China)
  • Received:2017-04-14 Revised:2017-05-09 Online:2017-06-30 Published:2017-06-30
  • Contact: LI Xiu-ping

摘要: 以卷丹百合Lilium lancifolium鳞片为外植体,采用1/2MS + 0.8 mg·L-1 6-BA + 0.2 mg·L-1 NAA为基本培养基,研究不同消毒时间的外植体消毒效果,探讨聚乙烯吡咯烷酮(PVP)、抗坏血酸(V?)、活性炭(AC)以及光、暗两种培养条件对卷丹百合组织培养过程中褐变的影响。结果表明,用75%酒精消毒30 s、0.1% HgCl2消毒10 min、2% NaClO消毒6 min的消毒效果最好,污染率为33.33%,成活率和诱导率均为66.67%。在培养基中加入PVP、V?、AC和暗培养均能减轻褐变的发生,其中以4.5 mg·L-1 PVP处理对卷丹百合褐变的抑制效果最好,外植体褐变率最低为30%,愈伤组织诱导率为70%。

关键词: 卷丹百合, 鳞片, 褐变

Abstract: Lilium lancifolium bulb scales as explants, 1/2MS+ 0.8 mg·L-1 6-BA+ 0.2 mg·L-1 NAA as the basic medium, the effects of different time on disinfection and the impact on browning with polyvinylpyrrolidone (PVP), ascorbic acid(V?), activated carbon (AC), light and dark treatments were studied. The results showed that there were two disinfection methods having the best effect, one was 75% ethanol for 30 s, followed by 0.1% HgCl2 for 10 min, the other was 2% NaClO for 6 min, the contamination rates of both were 33.33%, the survival rate and callus induction rate of both were 66.67%. Browning could be reduced with the medium involved in polyvinylpyrrolidone (PVP), V?, activated carbon, and in the dark conditions. The best effect of browning was 4.5 mg·L-1 PVP, the browning rate was 30%, callus induction rate was up to 70%.

Key words: Lilium lancifolium, bulb scale, browning

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