重瓣荷花内、外被花色、酚类物质及抗氧化活性比较分析

doi:10.3969/j.issn.1009-7791.2026.03.005

亚热带植物科学 ›› 2026, Vol. 55 ›› Issue (3): 305-314.DOI: 10.3969/j.issn.1009-7791.2026.03.005

重瓣荷花内、外被花色、酚类物质及抗氧化活性比较分析

张厚琛^1,2，廖丽秀^1,2，田代科²，赵雨萌^2,3，霍晶晶^2,4，付乃峰²，张大生^2*，刘青青^2*

(1. 吉首大学生命科学学院，湖南吉首 416000；2. 上海辰山植物园 / 上海市资源植物功能基因组学重点实验室，上海 201602；3. 宁夏大学生命科学学院，宁夏银川 750021；4. 上海师范大学生命科学学院，上海 200234)

收稿日期:2026-03-20 接受日期:2026-05-12 出版日期:2026-06-30 发布日期:2026-06-30
通讯作者: 张大生; 刘青青
基金资助:
国家自然科学基金项目(32502763)；上海市科学技术委员会项目(25ZR1402139)；上海市绿化和市容管理局项目(G252414、G262409、G242405)

Comparison of Flower Color, Phenolic Compounds, and Antioxidant Activity between Inner and Outer Tepals of Double-Flowered Lotus

ZHANG Hou-chen¹, LIAO Li-xiu^1,2, TIAN Dai-ke², ZHAO Yu-meng^2,3, HUO Jing-jing^2,4, FU Nai-feng², ZHANG Da-sheng^2*, LIU Qing-qing^2*

(1. College of Life Sciences, Jishou University, Jishou 416000, Hunan China; 2. Shanghai Key Laboratory of Plant Functional Genomics and Resources / Shanghai Chenshan Botanical Garden, Shanghai 201602, China; 3. College of Life Sciences, Ningxia University, Yinchuan 750021, Ningxia China; 4. College of Life Sciences, Shanghai Normal University, Shanghai 200234, China)

Received:2026-03-20 Accepted:2026-05-12 Online:2026-06-30 Published:2026-06-30
Contact: ZHANG Da-sheng; LIU Qing-qing

摘要/Abstract

摘要： 为探究重瓣荷花内、外被花色表型、酚类物质积累及抗氧化活性的差异及内在关联，选取7个不同花色重瓣荷花品种，对其内、外被进行系统性比较分析。采用分光测色仪测定花色表型参数，通过HPLC-QE-MS/MS定性定量分析类黄酮及花青素苷成分，利用分光光度法检测总酚含量，借助ABTS法评估总抗氧化活性，并系统分析各指标在内、外被间的差异及其相关性。结果表明，7个品种荷花内、外被共检测到13种黄酮醇和5种花青素苷，其中槲皮素苷与山奈酚苷为核心类黄酮组分，二者占比高于85%。内、外被在花色参数、类黄酮组成及总酚含量上均呈现明显的部位特异性。相关性分析显示，内、外被总酚含量的差异与其抗氧化活性的差异呈显著正相关，表明酚类物质的空间分布差异是荷花内、外被抗氧化活性分化的重要物质基础。花色参数的差异与总酚、总黄酮醇及多种黄酮醇类成分的含量差异存在不同程度相关性。本研究系统分析重瓣荷花内、外被花色、酚类物质及抗氧化活性差异，明确功能成分在内、外被间的部位差异性积累及其与抗氧化功能的关系，为荷花花色育种及以花瓣为导向的功能性资源利用提供理论依据。

关键词: 荷花, 重瓣花, 花色, 类黄酮, 抗氧化活性

Abstract: Seven cultivars of double-flowered lotus with different petal colors are used for exploring differences in floral color phenotype, phenolic accumulation, and antioxidant function between the inner and outer tepals of flowers. Floral color parameters were measured using a spectrophotometer; flavonoids and anthocyanins were qualitatively and quantitatively analyzed by HPLC-QE-MS/MS; total phenolic content was determined by spectrophotometry; and total antioxidant activity was evaluated by the ABTS method. The differences and correlations of these indices between inner and outer tepals were systematically analyzed. The results showed that 13 flavonols and 5 anthocyanins were detected in the inner and outer tepals of seven lotus cultivars, among which quercetin glycosides and kaempferol glycosides accounting for more than 85% were the core flavonoid components. The inner and outer tepals exhibited obvious site-specific differences in floral color parameters, flavonoid composition, and total phenolic content. Correlation analysis indicated that the difference in total phenolic content between inner and outer tepals was significantly positively correlated with the difference in antioxidant activity, suggesting that the spatial distribution difference of phenolic compounds is an important material basis leading to the differentiation of antioxidant activity in lotus inner and outer tepals. Differences in tepal color parameters were correlated with variations in total phenolics, total flavonols, and various flavonol components. This study systematically compared the differences in floral color, phenolic compounds, and antioxidant activity between inner and outer tepals of double-flowered lotus, clarified the differential accumulation of functional components and their relationship with antioxidant function in inner and outer tepals, and provides a theoretical basis for lotus floral color breeding and petal-oriented functional resource utilization.

Key words: lotus, double-flowered, flower color, flavonoids, antioxidant activity

中图分类号:

Q946

张厚琛, 廖丽秀, 田代科, 赵雨萌, 霍晶晶, 付乃峰, 张大生, 刘青青. 重瓣荷花内、外被花色、酚类物质及抗氧化活性比较分析[J]. 亚热带植物科学, 2026, 55(3): 305-314.

ZHANG Hou-chen, LIAO Li-xiu, TIAN Dai-ke, ZHAO Yu-meng, HUO Jing-jing, FU Nai-feng, ZHANG Da-sheng, LIU Qing-qing. Comparison of Flower Color, Phenolic Compounds, and Antioxidant Activity between Inner and Outer Tepals of Double-Flowered Lotus[J]. Subtropical Plant Science, 2026, 55(3): 305-314.

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重瓣荷花内、外被花色、酚类物质及抗氧化活性比较分析

Comparison of Flower Color, Phenolic Compounds, and Antioxidant Activity between Inner and Outer Tepals of Double-Flowered Lotus

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