关键词: 盐酸小糪碱, 绿原酸, 薄层色谱, 高效液相色谱

Abstract: To establish the research method for compound prescription cortex phellodendri chinensis fluid, the berberine hydrochloride and chlorogenic acid in compound prescription cortex phellodendri chinensis fluid were tested by the method of TLC and HPLC. The results showed that spots obtained from the sample solutions had the same color as their authentic sample solutions, the blank solutions had no interference in TLC of compound prescription cortex phellodendri chinensis fluid. The content of berberine hydrochloride in the range of 0.09 to 0.45 μg had good linear relationship, the average recovery was 103.4%; the content of chlorogenic acid in the range of 0.9 to 4.5 μg had good linear relationship, the average recovery was 100.3%. In conclusion, this method can be used in the quality control of compound cortex phellodendri chinensis fluid.

Key words: berberine hydrochloride, chlorogenic acid, TLC, HPLC