Subtropical Plant Science ›› 2005, Vol. 34 ›› Issue (02): 41-42,45.

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Measurement of Ursolic Acid Content in Clerodendranthus spicatus

ZHANG Ping1, HU Tan-lian2, LUO Guan-xing1, TIE Wan-zhu1, LAN Shi-kuan1   

  1. 1. Institute of Subtropical Crops, Liangshan Prefecture of Sichuan Province, Miyi 617201, Sichuan China;
    2. Pharmacential College, Chengdu University of Tradition Chinese Medicine, Chengdu 610032, Sichuan China
  • Received:2004-12-13 Online:2005-06-10 Published:2005-06-10

肾茶熊果酸含量测定的研究

张平1,胡坦莲2,罗关兴1,铁万祝1,兰世宽1   

  1. 1. 四川省凉山州 亚热带作物研究所, 四川 米易 617201;
    2. 成都中医药大学 药学院, 四川 成都 610032
  • 作者简介:张平(1971-),男,四川富顺人,农艺师,从事南药栽培研究与开发推广工作。
  • 基金资助:

    国家级星火计划项目(97B101D8100017)资助(子课题)

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Abstract: A TLCS method for determining the content of ursolic acid in Clerodendranthus spicatus was established in this experiment. The method was as follows. After extraction, the samples were dripped on silica gel GF254 TLC plate, using a mixture of the alkane-chloroform-acetic acid B ester-methyl alcohol (25:15:5:2) as developer and locating the position under the light of an UV lamp(254nm); double wavelength reflection sawtoothed scanning was performed λS=520nm, λR=650nm. The results showed that the regression equation is y=3658.34 X-7857.07, r=0.9998 and linear range is 0.5~2.5μg. The average recovery rate is 101.57%, RSD=2.83%.

Key words: Clerodendranthus spicatus, ursolic acid, TLC method, content measurement

摘要： 本试验研究用薄层扫描法测定肾茶中熊果酸含量。样品经适当提取后点于硅胶GF254薄层板上,以环已烷-氯仿-乙酸乙酯-甲醇(25:15:5:2)为展开剂,置于紫外光灯(波长254nm)下定位;双波长反射锯齿扫描,λS=520nm,λR=650nm。结果表明,经方法学考察,点样量在0.5~2.5μg范围内呈现良好的线性关系,其回归方程为y=3658.34X-7857.07,r=0.9998;平均回收率为101.57%,RSD=2.83%。

关键词: 肾茶, 熊果酸, 薄层扫描法, 含量测定

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