Abstract: Dicranopteris dichotoma polysaccharide was extracted by four methods,among those that the extracting rate of synthesis method was the highest,reaching 5.2%. The crude polysaccharide was purified by DEAE-52 and the neutral polysaccharide was eluted by distilled water and the polyanion polysaccharide was gradually eluted by 0.8mol/L NaCl. Their contents were 78% and 22%, respectively. The antibiotic activities of Dicranopteris dichotoma were tested with the agar disc diffusion method. The result showed that the polysaccharide had various amount of antibiotic activities against bacteria and fungi.

Key words: Dicranopteris dichotoma, polysaccharide, extraction, isolation, antibiotic activities

摘要： 采用4种方法提取芒萁多糖,其中综合法提取率为5.2%;可溶性多糖经DEAE-纤维素52柱层析进一步纯化,得到1种中性多糖和1种酸性多糖,各占78%和22%。利用纸片法进行抑菌实验结果表明,芒萁多糖显示了不同程度抑制细菌和真菌的活性。

关键词: 芒萁, 多糖, 提取, 分离, 抗菌活性