Identification and Expression Analysis of the Dof Gene
Family in Eucalyptus grandis

doi:10.3969/j.issn.1009-7791.2026.01.001

Subtropical Plant Science ›› 2026, Vol. 55 ›› Issue (1): 1-11.DOI: 10.3969/j.issn.1009-7791.2026.01.001

• Research articles • Next Articles

Identification and Expression Analysis of the Dof Gene Family in Eucalyptus grandis

YANG Yu-ting, FENG Zi-yi, WU Ai-min^*

(College of Forestry and Landscape Architecture, South China Agricultural University / Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, Guangdong China)

Received:2025-09-20 Accepted:2025-12-26 Online:2026-02-28 Published:2026-03-01
Contact: WU Ai-min

巨桉Dof基因家族鉴定及表达分析

杨玉婷，冯子怡，吴蔼民^*

(华南农业大学林学与风景园林学院 / 广东省森林植物种质创新与利用重点实验室，广东广州 510642)

通讯作者: 吴蔼民
基金资助:
科技部科技创新2030-重大项目(2023ZD0405702-03)

Abstract

Abstract: The Dof (DNA binding with one finger) family of transcription factors is a special kind found in plants and is crucial for biological processes including photomorphogenesis, stress response, and plant growth and development. This research focuses on the Eucalyptus grandis Dof gene family, systematically analyzing its evolutionary relationships, gene structure, cis-acting elements, member identification, and synteny analysis. According to the findings, 26 members of the Dof gene family were found in the E. grandis genome. These members generally reside in the nucleus, with protein lengths ranging from 183 to 516 amino acids, and an isoelectric point that is often higher than 7.0. According to chromosomal location, all proteins have the characteristic Dof zinc finger domain, and the 26 EgrDof genes are widely dispersed over 10 chromosomes. According to an analysis of the cis-acting regions in the promoters, abiotic stress regulation, hormone response, and light sensing are all extensively impacted by genes. EgrDofs expression levels are higher in stems and phloem, while levels are comparatively low in leaves at various development stages. This study offers a strong basis for comprehending the regulatory networks and biological functions of Dof genes.

Key words: Eucalyptus grandis, Dof transcription factor; bioinformatics, tissue expression

摘要： Dof (DNA binding with one finger)家族作为植物中特有的转录因子，在植物生长发育、光形态建成以及逆境响应等生物学过程中具有重要作用。本研究基于巨桉Eucalyptus grandis Dof基因家族，系统分析了其家族成员、进化关系、基因结构、顺式作用元件及共线性分析。结果显示，在巨桉的基因组中鉴定出26个Dof基因家族成员，其蛋白质长度在183到516个氨基酸之间，等电点普遍大于7.0，且多数定位于细胞核。染色体定位显示26个EgrDofs基因不均匀分布在10条染色体上，且所有蛋白均含典型Dof锌指结构域。启动子顺式作用元件分析揭示，EgrDofs基因广泛参与光感应、激素响应和非生物胁迫调控。EgrDofs在不同生长时间段的叶片表达量较低，在茎和韧皮部中表达量较高。该研究可为Dof基因的生物学功能和调控网络提供参考基础。

关键词: 巨桉, Dof转录因子, 生物信息学, 表达分析

CLC Number:

Q943.2

YANG Yu-ting, FENG Zi-yi, WU Ai-min. Identification and Expression Analysis of the Dof Gene Family in Eucalyptus grandis[J]. Subtropical Plant Science, 2026, 55(1): 1-11.

杨玉婷, 冯子怡, 吴蔼民. 巨桉Dof基因家族鉴定及表达分析[J]. 亚热带植物科学, 2026, 55(1): 1-11.

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Identification and Expression Analysis of the Dof Gene Family in Eucalyptus grandis

巨桉Dof基因家族鉴定及表达分析

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