Stoichiometric Characteristics and Correlations of Silage Maize-Soil
under Water-Nitrogen Coupling in Hexi Corridor

doi:10.3969/j.issn.1009-7791.2026.03.008

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Subtropical Plant Science ›› 2026, Vol. 55 ›› Issue (3): 336-350.DOI: 10.3969/j.issn.1009-7791.2026.03.008

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Stoichiometric Characteristics and Correlations of Silage Maize-Soil under Water-Nitrogen Coupling in Hexi Corridor

LI Chun-yu, MA Wei-wei^*, ZHU Niu, WANG Jian-fang, LI Guang, WU Jiang-qi

(College of Forestry, Gansu Agricultural University, Lanzhou 730070, Gansu China)

Received:2025-12-07 Accepted:2026-02-11 Online:2026-06-30 Published:2026-06-30
Contact: MA Wei-wei

河西地区水氮耦合下青贮玉米-土壤化学计量特征及其相关性

李春宇，马维伟^*，朱牛，汪建芳，李广，吴江琪

(甘肃农业大学林学院，甘肃兰州 730070)

通讯作者: 马维伟
基金资助:
甘肃省教育厅产业支撑计划项目(2025CYZC-042)；中央引导地方科技发展基金项目(24ZYQA023)；甘肃省拔尖领军人才项目(GSBJLJ-2023-09)；甘肃省青年博士支持项目(2025QB-043)

Abstract

Abstract: The Hexi Corridor is a critical base for forage crop agriculture in northwestern China. In order to explore the stoichiometric characteristics of carbon (C), nitrogen (N), and phosphorus (P) in plants and soil of silage maize under varying water and nitrogen conditions in the Hexi region, as well as their influencing factors, a two-factor water-nitrogen experiment was conducted. Based on local irrigation and fertilization practices, three nitrogen application levels (0 (N0), 311 (N1), and 280 (N2) kg·hm^–2) and three irrigation levels (6765 (W1), 6150 (W2), and 5535 (W3) m³·hm^–2) were established. The contents of C, N, and P in different maize organs and soil under water-nitrogen coupling were measured, along with agronomic traits such as dry matter mass of roots, stems, and leaves, plant height, stem diameter, leaf area, soil nutrients, and microbial enzyme activities. The stoichiometric characteristics of soil-plant systems and their influencing factors under different treatments were investigated. The results showed that water-nitrogen coupling significantly affected maize growth. The W3N2 treatment achieved the highest plant height at maturity (322.72 cm), while the W2N2 treatment produced the maximum biomass at maturity (414.64 g). Leaves exhibited the highest N and P contents, and plant N:P decreased as the growth period advanced. Soil organic carbon (SOC) and total nitrogen (TN) were higher at the jointing stage, and enzyme activities were regulated by water-nitrogen treatments. The correlation between aboveground and belowground parts strengthened with growth progression, being most significant at the jointing and silking stages. At the silking stage, plant N:P showed a highly significant positive correlation with soil N:P, representing one of the key stages with the closest association between the two throughout the growth period. This study provides scientific support for the development of silage maize production in the Hexi Oasis, contributes to theoretical guidance for water-nitrogen resource-saving agriculture in the Hexi Oasis irrigation area, and offers references for water and fertilizer management of silage maize in arid regions.

Key words: water-nitrogen coupling, soil nutrients, growth stage, stoichiometric characteristics

摘要： 河西走廊是西北地区饲草农业发展的重要基地。为探究河西地区青贮玉米在不同水肥条件下植物-土壤C、N、P化学计量特征及其影响因素，采用双因素水氮试验，在结合当地经验灌溉和施肥的基础上，分别设置3种施氮水平[0 (N0)、311 (N1)、280 (N2) kg·hm^–2]以及三个灌溉水平[6765 (W1)、6150 (W2)、5535 (W3) m³·hm^–2]。通过测定水氮耦合下玉米不同器官及土壤碳（C）、氮（N）、磷（P）元素含量，以及根、茎、叶干物质量、株高、茎粗、叶面积等农艺性状，土壤养分和微生物酶活性，研究不同处理下土壤-植物化学计量特征及其影响因素。结果表明，水氮耦合显著影响玉米生长， W2N2处理为综合最优水氮组合，该处理下玉米成熟期生物量达 414.64 g、株高 300.75 cm，叶片氮磷含量协调；土壤有机碳、全氮含量及脲酶、碱性磷酸酶活性维持较高水平，实现作物高产与土壤健康的协同。植株叶片氮磷含量最高，植株N:P随生育期推进下降；土壤有机碳（SOC）、总氮（TN）在拔节期较高，酶活性受水氮处理调控；地上-地下相关性随生育期增强，拔节期和吐丝期最显著，在吐丝期，植株N:P与土壤N:P呈现极显著正相关关系，是整个生育期中两者关联最为紧密的关键阶段之一。

关键词: 水氮耦合, 土壤养分, 生育期, 化学计量特征

LI Chun-yu, MA Wei-wei, ZHU Niu, WANG Jian-fang, LI Guang, WU Jiang-qi. Stoichiometric Characteristics and Correlations of Silage Maize-Soil under Water-Nitrogen Coupling in Hexi Corridor[J]. Subtropical Plant Science, 2026, 55(3): 336-350.

李春宇, 马维伟, 朱牛, 汪建芳, 李广, 吴江琪. 河西地区水氮耦合下青贮玉米-土壤化学计量特征及其相关性[J]. 亚热带植物科学, 2026, 55(3): 336-350.

References

[1] Li L J, Cheng X G, Zhang Y, Kohtz D, Wang X H, Zhang X T, Kong X J, Xue H Y, Jia P P, Bai N, Li Z Q, Xiao P, Pan X P, Zhang Z Y, Zhang B H. Exogenous melatonin improves peanut field productivity and quality at reduced nitrogen application [J]. Field Crops Research, 2024, 319: 109650.

[2] Elser J J, Fagan W F, Kerkhoff A J, Swenson N G, Enquist B J. Biological stoichiometry of plant production: metabolism, scaling and ecological response to global change [J]. New Phytologist, 2010, 186(3): 593–608.

[3] 李玉霖, 毛伟, 赵学勇, 张铜会. 北方典型荒漠及荒漠化地区植物叶片氮磷化学计量特征研究[J]. 环境科学, 2010, 31(8): 1716–1725.

[4] Wang J J, Liang Y Y, Wang G A, Lin X Y, Liu J X, Wang H, Chen Z X, Wu B S. Leaf nitrogen and phosphorus stoichiometry and its response to geographical and climatic factors in a tropical region: evidence from Hainan Island [J]. Agronomy, 2023, 13(2): 411.

[5] 李志强, 齐鹏, 王雅芝, 王晓娇, 甘润, 薛江龙, 李国山. 氮磷添加对黄土高原小麦土壤养分含量及生态化学计量特征的影响[J]. 中国农学通报, 2024, 40(10): 95–102.

[6] Zhang L, Yan M F, Li H B, Ren Y Y, Siddique K H M, Chen Y L, Zhang S Q. Effects of zinc fertilizer on maize yield and water-use efficiency under different soil water conditions [J]. Field Crops Research, 2020, 248: 107718.

[7] Xing Y Y, Li Y, Zhang F, Wang X K. Appropriate application of organic fertilizer can effectively improve soil environment and increase maize yield in Loess Plateau [J]. Agronomy, 2024, 14(5): 993.

[8] Tolera A, Sundstøl F, Said A N. The effect of stage of maturity on yield and quality of maize grain and stover [J]. Animal Feed Science and Technology, 1998, 75(2): 157–168.

[9] Zhan X Y, Zhang Q W, Zhang H, Hussain H A, Shaaban M, Yang Z L. Pathways of nitrogen loss and optimized nitrogen management for a rice cropping system in arid irrigation region, northwest China [J]. Journal of Environmental Management, 2020, 268: 110702.

[10] 中华人民共和国农业农村部. 农业农村部关于印发《“十四五”全国种植业发展规划》的通知[J]. 中华人民共和国农业农村部公报, 2022 (2): 26–43.

[11] Wang L, Chen R S, Han C T, Wang X Q, Liu G H, Song Y X, Yang Y, Liu J F, Liu Z W, Liu X J, Guo S H, Zheng Q. Change characteristics of precipitation and temperature in the Qilian Mountains and Hexi Oasis, Northwestern China [J]. Environmental Earth Sciences, 2019, 78(9): 284.

[12] 谢明君, 李广, 马维伟, 刘帅楠, 常海刚, 杜佳囝, 丁宁. 水分对河西青贮玉米土壤化学计量比及稳态性的影响[J]. 干旱区研究, 2022, 39 (4): 1312–1321.

[13] 张建喜, 李广, 王钰煐, 袁建钰, 徐国荣, 张武清. 水氮协同对河西走廊青贮玉米土壤氮含量及酶活性的影响[J]. 中国土壤与肥料, 2024(10): 16–25.

[14] Zhang J, Guan Q Y, Zhang Z P, Shao W Y, Zhang E, Kang T T, Xiao X, Liu H Q, Luo H P. Characteristics of spatial and temporal dynamics of vegetation and its response to climate extremes in ecologically fragile and climate change sensitive areas – A case study of Hexi region [J]. CATENA, 2024, 239: 107910.

[15] 王丙生, 陆燕花, 李广, 谢洋, 朱莹莹, 谈燕. 减氮和配施腐植酸对河西灌区青贮玉米生长、土壤有机碳组分及碳库管理指数的影响[J/OL]. 草原与草坪. https://link.cnki.net/urlid/62. [2025–12–04].

[16] Fu J, Niu J, Kang S Z, Adeloye A J, Du T S. Crop production in the Hexi Corridor challenged by future climate change [J]. Journal of Hydrology, 2019, 579: 124197.

[17] 秦子元, 张忠学, 杜思澄, 黄彦, 王柏, 张作合. 水氮耦合下黑土稻作碳氮磷累积分配和化学计量特征[J]. 农业机械学报, 2022, 53(10): 326–339.

[18] Yu T F, Hao F, Gao K, Xiong M, An H B. Productivity, soil fertility and enzyme activity of mixed forage grasslands improved by alfalfa and nitrogen addition in Horqin Sandy Land, China [J]. Scientific Reports, 2025, 15(1): 10748.

[19] 宋良翠, 马维伟, 李广, 龙永春, 常文华. 水分对尕海湿地退化演替土壤氮矿化的影响[J]. 干旱区研究, 2022, 39(1): 165–175.

[20] 李佳旺, 徐嘉, 于航, 罗慧, 沈菊培, 陈超, 白鑫, 岳中辉. 土壤脲酶酶促反应对3种有机氮肥施用的响应[J]. 中国生态农业学报(中英文), 2024, 32(6): 1033–1042.

[21] Thanapon C, Karan W, Manatsanan P, Piamsook P, Rath P. Energy– and evolution–based design of inulosucrase for enhanced thermostability and inulin production [J]. Applied microbiology and Biotechnology, 2023, 107(22): 6831–43.

[22] 卢研, 张倩, 张彩军, 孙小妹, 苏军虎. 生物土壤结皮对东祁连山高寒草甸土壤特性的影响[J]. 草原与草坪, 2022, 42(1): 13–20.

[23] 王春莲, 鞠方成, 赵方奎, 丁树启. 氮肥配施生物炭对土壤酶活性及春玉米产量的影响[J]. 辽东学院学报(自然科学版), 2025, 32 (1): 23–31.

[24] 郭长春, 徐富贤, 周兴兵, 贾敏, 郭晓艺, 蒋鹏, 朱永川, 刘茂, 田绍平, 张林. 施氮对酿酒专用稻产量及品质的影响[J]. 作物研究, 2025, 39(3): 193–198.

[25] Zhang F, Chen M R, Xing Y Y, Wang X K. Blended urea application improves spring maize productivity and soil quality, especially under ridge-furrow plastic film mulching planting [J]. European Journal of Agronomy, 2026, 172: 127877.

[26] Yang T R, Zhao J H, Hong M, Ma M J. Appropriate water and nitrogen supply regulates the dynamics of nitrogen translocation and thereby enhancing the accumulation of nitrogen in maize grains [J]. Agricultural Water Management, 2024, 306: 109160.

[27] Shen F Y, Fei L J, Peng Y L, Gao Y L. Temperature-driven regulatory mechanism of dry matter accumulation and yield optimization in summer maize under mulched water-nitrogen coupling [J]. Journal of Agriculture and Food Research, 2025. https://doi.org/10.1016/j.jafr.2025.102442

[28] 戚迎龙, 宁小莉, 孙贯芳, 李敏, 史海滨. 水氮耦合对滴灌玉米光合特性及产量的调控效应[J]. 东北农业大学学报, 2024, 55(3): 10–20.

[29] 申祺, 于小彭, 李蔚然, 王康健, 展广军, 于艳青. 水氮耦合对水稻生长发育、碳氮代谢和氮吸收利用的调控研究进展[J]. 北方水稻, 2025, 55(4): 47–50.

[30] 李燕楠, 张振宇, 秦树平, 李晓欣, 李红军, 张玉铭, 胡春胜, 张丽娟, 董文旭, 刘秀萍, 郑普山. 长期施氮耦合短期玉米间作豆科作物对土壤团聚体组成及碳氮分布的影响[J]. 中国生态农业学报(中英文), 2025, 33(9): 1831–1846.

[31] Tian X, Zhang C, Ju X T. Crop responses to application of optimum nitrogen fertilizers on soils of various fertilities formed from long-term fertilization regimes [J]. European Journal of Agronomy, 2023, 148: 126857.

[32] 樊吴静, 杨鑫, 何虎翼, 唐洲萍, 唐美丽, 韦玉恒, 李丽淑. 水氮耦合对旱藕产量、生理特性和土壤理化性状的影响[J]. 西南农业学报, 2022, 35(5): 1069–1078.

[33] Bian Z H, Tian H Q, Pan S F, Shi H, Lu C Q, Anderson C, Cai W J, Hopkinson C S, Justic D, Kalin L. Soil legacy nutrients contribute to the decreasing stoichiometric ratio of N and P loading from the Mississippi River Basin [J]. Global Change Biology, 2023, 29(24): 7145–58.

[34] Liu L J, Zhu Q L, Wen D N, Yang L, Ni K, Xu X L, Cao J H, Meng L, Yang J L, Zhou J X. Stimulation of organic N mineralization by N-acquiring enzyme activity alleviates soil microbial N limitation following afforestation in subtropical karst areas [J]. Plant and Soil, 2024, 504(1): 879–94.

[35] Li D X, Zhang T T, Yu H H, Li Y, Lv T, Yu D, Liu C H. The impacts of different nitrogen supply on root traits, root exudates, and soil enzyme activities of exotic and native plant communities [J]. Plant and Soil, 2025, 508(1): 209–26.

[36] 丁颖, 刘小伟, 郭梁, 景煜都, 封俊豪, 梁可, 裴梦婷, 熊千里. 黄土高原草地灌丛化对土壤有机碳、氮磷养分及酶活性的影响[J].水土保持通报, 2024, 44(5): 243–250,261.

[37] Li C, Liu L, Zheng L, Yu Y, Mushinski R M, Zhou Y, Xiao C W. Greater soil water and nitrogen availability increase C: N ratios of root exudates in a temperate steppe [J]. Soil Biology and Biochemistry, 2021, 161: 108384.

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Stoichiometric Characteristics and Correlations of Silage Maize-Soil under Water-Nitrogen Coupling in Hexi Corridor

河西地区水氮耦合下青贮玉米-土壤化学计量特征及其相关性

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