花榈木组培苗茎段低温胁迫培养及耐冷性诱导

doi:10.3969/j.issn.1009-7791.2009.02.005

亚热带植物科学 ›› 2009, Vol. 38 ›› Issue (02): 19-21,25.DOI: 10.3969/j.issn.1009-7791.2009.02.005

花榈木组培苗茎段低温胁迫培养及耐冷性诱导

高丽,杨波,李洪林

中国科学院武汉植物园, 湖北武汉 430074

收稿日期:2008-12-03 出版日期:2009-06-10 发布日期:2009-06-10
作者简介:高丽(1979-),女,河南南阳人,硕士,从事植物生物技术研究。
基金资助:
中国科学院知识创新工程重要方向性项目(KSCX2-YW-N-032)资助

Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro

GAO Li, YANG Bo, LI Hong-lin

Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei China

Received:2008-12-03 Online:2009-06-10 Published:2009-06-10

摘要/Abstract

摘要： 以花榈木组培苗茎段为试材,在低温胁迫条件下进行耐冷性愈伤组织诱导培养。结果表明,5℃是花榈木茎段低温胁迫培养及耐冷性诱导的最适温度。经过5℃低温胁迫培养获得的耐冷性愈伤组织在分化培养基MS+6-BA 2.0 mg/L+NAA 0.25 mg/L上的分化率达70.0%,且再生植株的低温伤害率明显低于对照。

关键词: 花榈木, 低温胁迫, 耐冷性

Abstract: Stems of tissue culture seedlings of Ormosia henryi were cultured under low temperature stress. The results showed that the optimal temperature for induction of chilling tolerance callus was 5℃. The differentiation frequency of chilling tolerance callus cultured under 5℃ was 70.0% on MS solid medium supplemented with 6-BA 2.0 mg/L and NAA 0.25 mg/L, and the injury rate of regenerated plants from chilling tolerant callus was much lower than that of control group.

Key words: Ormosia henryi, low temperature stress, chilling tolerance

中图分类号:

Q945.78

高丽,杨波,李洪林. 花榈木组培苗茎段低温胁迫培养及耐冷性诱导[J]. 亚热带植物科学, 2009, 38(02): 19-21,25.

GAO Li, YANG Bo, LI Hong-lin. Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro[J]. Subtropical Plant Science, 2009, 38(02): 19-21,25.

[1]	池敏杰，刘育梅. 3种番荔枝属植物对低温胁迫的生理响应及抗寒性评价[J]. 亚热带植物科学, 2019, 48(04): 339-342.
[2]	周强,韩颖颖. 植物低温应答机制及其泛素化修饰研究进展[J]. , 2017, 46(02): 195-200.
[3]	陈淳,陈丽璇,柯合作,陈丽虹,刘黎卿. 低温胁迫对建兰叶片内源多胺含量的影响[J]. 亚热带植物科学, 2010, 39(02): 1-4.

花榈木组培苗茎段低温胁迫培养及耐冷性诱导

Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics