亚热带植物科学 ›› 2014, Vol. 43 ›› Issue (02): 108-109.DOI: 10.3969/j.issn.1009-7791.2014.02.004

• 生理生化 • 上一篇    下一篇

有柄石韦总多酚含量测定及其超声提取工艺优化

程丹丹,高贝,辛晓伟,高德民   

  1. (山东中医药大学 药学院,山东 济南 250355)
  • 收稿日期:2014-03-06 修回日期:2014-03-06 出版日期:2014-06-10 发布日期:2014-06-10
  • 通讯作者: 高德民
  • 作者简介:程丹丹,硕士研究生,从事中药资源与质量控制研究。
  • 基金资助:

    山东中医药大学2013年SRT

Polyphenols Extraction from Pyrrosia petiolosa by Ultrasonic and Its Content Determination

CHENG Dan-dan,GAO Bei,XIN Xiao-wei,GAO De-min   

  1. (School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong China)
  • Received:2014-03-06 Revised:2014-03-06 Online:2014-06-10 Published:2014-06-10
  • Contact: GAO De-min

摘要: 为优选有柄石韦Pyrrosia petiolos中总多酚超声提取工艺,采用Folin-Ciocalteus比色法对总多酚进行含量测定,通过单因素和正交试验,对提取工艺参数进行优化。结果表明,没食子酸对照品在浓度为1~10 mg·L-1范围内,与吸光度呈良好线性关系(y = 2.406x-0.012,R = 0.9994),有柄石韦总多酚的最佳提取工艺条件为:55%乙醇,料液比1∶10(W/V),超声提取60 min,超声提取温度75 ℃,总多酚提取率为21.47 mg·g-1

关键词: 超声法, 有柄石韦, 总多酚, 提取工艺, 正交试验

Abstract: To optimize the extraction of total polyphenols in Pyrrosia petiolosa by ultrasonic, the content of the polyphenols was used as investigation index, and the total polyphenol content was determined by Folin-Ciocalteus colorimetric. The extraction process parameters for polyphenols were optimized by the single factor and the orthogonal experiment. The results showed that there was a good linearity between gallic acid concentration and absorbance in the range 1~10 mg·L-1 (y = 2.406x -0.012, R= 0.9994);The optimum conditions were as follows: solvent concentration 55%, ultrasound time 60 min, extraction temperature 75 ℃, solid to liquid ratio 1∶10. The polyphenols extraction rate was 21.47 mg·g-1 in the best technical conditions.

Key words: ultrasonic, Pyrrosia petiolosa, polyphenols, extracting technique, orthogonal experiment

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