四棱豆根瘤菌的分离及特性

亚热带植物科学 ›› 1990, Vol. 19 ›› Issue (02): 17-21.

四棱豆根瘤菌的分离及特性

蓝谷,蔡龙祥,黄维南

福建省亚热带植物研究所, 厦门 361009

出版日期:1990-06-10 发布日期:1990-06-10
基金资助:
福建省自然科学基金资助

ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM Psophocarpus tetragonolobus

Lan Gu, Cai Longxiang, Huang Weinan

Fujian Institute of Subtropical Botany, Xiamen 361009

Online:1990-06-10 Published:1990-06-10

摘要/Abstract

摘要： 从四棱豆根瘤上分离出一株慢生型根瘤菌(Rhizolia Ps)。在显微镜下,该菌株为革兰氏阴性,有一根亚极生鞭毛,大小为0.59×1.49微米。其增代时间为15.52小时,在39℃和含1.5% NaCl培养基中均不能生长。回接时能使四棱豆幼苗根部结瘤,固氮酶活性为2.81微摩尔乙烯·克鲜瘤^-1·小时^-1。本文对该菌株的含碳化合物利用、B.T.B.反应、在肉膏蛋白胨上的生长情况、石蕊牛奶反应、淀粉和明胶水解等生理生化特征进行了试验。

关键词: 四棱豆, 根瘤菌特性

Abstract: A slow-growing Rhizcbium strain Ps was isolated from root nodules of Psophocarpus fetragonolobut. It was Gram-negative rod (0.59×1.49 μm) with subpolar flagella. Long doubling time (15.52 h) in YM liquid medium was observed. It did not grow both in 1.5% NaCl and at 39℃. When inoculated, nodules can be formed on the host plants. The ni-trogenase activity has been determined. Physiological and biclogical characteristics of strain Ps were systematically studied.

Key words: Psophocarpus tetragonolobus, Characteristics of rhizobium

蓝谷,蔡龙祥,黄维南. 四棱豆根瘤菌的分离及特性[J]. 亚热带植物科学, 1990, 19(02): 17-21.

Lan Gu, Cai Longxiang, Huang Weinan. ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM Psophocarpus tetragonolobus[J]. Subtropical Plant Science, 1990, 19(02): 17-21.