关键词: 赤豆, 酰脲, 尿囊素酶

Abstract: Changes of ureide, allantoin, allantoic acid content and al-lantoinase activity in adzuki bean (Vigna angularis) seedlings were investigated. Adzuki bean seeds were surface-sterilized in 0.1% HgCl₂ (w/v) for 2-3 min and then washed repeatedly with redistilled water, aseptically germinated in the redistilled water at 28±1℃ in the dark unless otherwise specified. The seedlings synthesized ureide (allantoin and allantoic acid) rapidly during the germination. There was little difference in the seedling ureide accumulation among cultiyars. Before the seedling ureide content reached the maximum, the content of allantoic acid was higher than that of allantoin in the seedling, and the ureide content of cotyledon increased more rapidly than that of any other parts in the seedlings. Adzuki bean allantoinase activity appeared on the first day of germination and reached the maximum on the fourth day. Allantoinase from adzuki bean seedlings was remarkably heat-stable and would retain about 60% of the original activity after a seventy-minute pretreatment at 70℃.

Key words: Adzuki bean, Ureide, Allantoinase